Adsorption Characteristics of Liquid Chromatography with Preparative Packings

제조용 충전물을 사용한 액체 크로마토그래피의 흡착특성

  • Received : 1998.02.11
  • Accepted : 1998.03.30
  • Published : 1998.06.10

Abstract

Preparative HPLC (High-Performance Liquid Chromatography) is mainly used for separating useful component from biological samples. By reversed-phase HPLC packed with preparative packings ($15{\mu}m$), the adsorption characteristics with sample size were investigated. Sample was 5'-GMP, a flavor enhancer, and the composition of mobile phase was 20mM $KH_2PO_4$ solution:methanol (97:3 vol.%). From the experimental results, the effect of sample size on retention factor was negligible, but the peak was asymmetrical above $1{\mu}g$ of sample. In addition, the increase in sample size deteriorated the number of theoretical plates, and at small concentration, the number of theoretical plates was less because of large peak width. In the experimental condition, the adsorption isotherm of 5'-GMP was relatively well represented by Freundlich equation.

Keywords

Acknowledgement

Supported by : 과학재단

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