Seasonal Changes of bacterial community analysed by fluorescent in situ hybridization method in Lake Soyang

Fluorescent In Situ Hybridization방법으로 분석한 소양호 세균 군집 구조의 계절적 변화

  • Hong, Sun-Hee (Dept. of Environmental Science, Kangwon National University, Research Center for Molecular Microbiology Seoul National University) ;
  • Ahn, Tae-Seok (Dept. of Environmental Science, Kangwon National University, Research Center for Molecular Microbiology Seoul National University)
  • 홍선희 (강원대학교 환경학과 및 서울대학교 분자미생물학연구센터) ;
  • 안태석 (강원대학교 환경학과 및 서울대학교 분자미생물학연구센터)
  • Received : 1998.07.30
  • Accepted : 1998.09.19
  • Published : 1998.09.01

Abstract

To define the structure and diversity of bacterial communities in the aqutic ecosystem, Lake Soyang, the largest artificial reservoir in Korea, a new method, fluorescent in situ hybridization was applied. This technique relies on the specific hybridization of the nucleic acid probes to the naturally amplified intracellular rRNA. By this method, the bacterial community composition of Lake Soyang and bacterial numbers belong to eubacteria, proteobacteria and Cytophaga-Flavobacterium group were estimated. Total bacterial numbers ranged from $0.3{\times}10^6{\sim}2.0{\times}10^6cells{\cdot}ml^{-1}$, and vertical profile of total bacteria showed the peak at 2 and 5 m depths. The ratio of eubacteria to total bacteria were 22~100% and varied with depth and season. The percentage of Proteobacteria ${\alpha}$-group ranged 2.6~66.7%, ${\beta}$-group 4.5~53.5%, ${\gamma}$-group 4.6~76.7% and Cytophaga-Flavobacterium group 2.1~35.9%. Also, bacteria] community had spatial and temporal characteristics. The dominant groups were ${\beta}$-group in winter, ${\gamma}$-group in spring and early summer and ${\alpha}$-group in summer.

Keywords

in situ hybridization;bacterial community;probe;Lake Soyang

Acknowledgement

Supported by : 한국 과학 재단