Demulsification of Petroleum Emulsion by Streptomyces sp. 8321

Streptomyces sp. 8321에 의한 석유 유상액의 탈유화

  • Ko, Sung-Hwan (Microbiology lab, Division of marine biology, Korea Ocean Research and Development Institute) ;
  • Lee, Deuk-Soo (Microbiology lab, Division of marine biology, Korea Ocean Research and Development Institute) ;
  • Kim, Sang-Jin (Microbiology lab, Division of marine biology, Korea Ocean Research and Development Institute) ;
  • Lee, Hong Kum (Microbiology lab, Division of marine biology, Korea Ocean Research and Development Institute)
  • 고성환 (한국해양연구소 해양생물부 미생물연구실) ;
  • 이득수 (한국해양연구소 해양생물부 미생물연구실) ;
  • 김상진 (한국해양연구소 해양생물부 미생물연구실) ;
  • 이홍금 (한국해양연구소 해양생물부 미생물연구실)
  • Received : 1998.06.08
  • Accepted : 1998.09.17
  • Published : 1998.09.01

Abstract

The characteristics of demulsification of petroleum emulsion by Streptomyces sp. 8321 were investigated. Demulsification ability of Streptomyces sp. 8321 appeared to be confined within the spores. Spore surface hydrophobicity was increased with culture age stimulating the demulsification ability. Over $1.1{\times}10^8spores/ml$ completely demulsified kerosene-0.2% Triton X-100 (2:1) emulsion. Among the low viscosity hydrocarbons, hydrocarbons with longer chain such as n-hexadecane and diesel were more rapidly demulsified. However, only 20-30% of the emulsion with high viscosity hydrocarbons was demulsified after 24 hours. Oil-in-water emulsions made by Corexit, Finalsol and BP series surfactants were completely demulsified within one minute. Demulsification rate ($t_{1/2}$) of oil-in-water emulsions made by Corexit 7664, 8667, Triton X-100 and Tween 80 decreased as their concentration increased. In case of water-in-oil emulsion made by Seagreen, $t_{1/2}$ was over 24 hours. Therefore, demulsification ability of Streptomyces sp. 8321 was more effective on oil-in-water emulsions.

Keywords

Demulsification;Hydrophobicity;Petroleum emulsion;Spore;Streptomyces sp.

Acknowledgement

Supported by : 과기부