Synthesis of Glycosides by Transglycosylation of α-Amylase from Starch

전분으로부터 α-amylase에 의한 배당체의 합성

  • Park, Jong Yi (Department of Microbiology, College of Natural Science, Pusan National University) ;
  • Lee, Tae Ho (Department of Microbiology, College of Natural Science, Pusan National University)
  • 박종이 (부산대학교 자연과학대학 미생물학과) ;
  • 이태호 (부산대학교 자연과학대학 미생물학과)
  • Received : 1998.05.18
  • Accepted : 1998.06.20
  • Published : 1998.09.01

Abstract

Glycosides were synthesized using transglycosylation reaction of amylase in water system. The glycosides synthesized in water phase by a-amylase with starch as a glycosyl donor and benzylalcohol as an acceptor were identified as benzylalcohol-${\alpha}$-glucoside (BG) and benzylalcohol-${\alpha}$-maltoside (BM) of which one molecule of benzylalcohol was bound to 1-OH of glucose. The final products were BG in reaction system of pH 5.0, and BM in that of pH 8.0. The transglycosylation reaction by ${\alpha}$-amylase were carried out in water system containing 50 mg starch, 50 mg benzylalcohol, and 10 units enzyme at $30-35^{\circ}C$ for 3 days. The synthesized BG was hydrolyzed to glucose and benzylalcohol by ${\alpha}$-glucosidase, while ${\alpha}$-amylase hydrolyzed BM to glucose and benzylalcohol-${\alpha}$-glucoside in pH 5.0. Maltotriose resemble structurally to BM was rapidly hydrolyzed to glucose and maltose by ${\alpha}$-amylase at pH 5.0, being slightly hydrolyzed at pH 8.0, but not transglycosylated in present of benzylalcohol.

Keywords

Transglycosylation;glycoside;benzylalcohol-${\alpha}$-glucoside

Acknowledgement

Supported by : 한국과학재단