Genetic Structure of the phnM Gene Encoding Plant-Type Ferredoxin from Pseudomonas sp. strain DJ77

Pseudomonas sp. strain DJ77에서 Plant-Type의 Ferredoxin을 암호화하는 phnM 유전자의 구조

  • Kim, Sungje (School of Life Sciences, Chungbuk National University) ;
  • Kim, Young-Chang (School of Life Sciences, Chungbuk National University)
  • Received : 1998.07.01
  • Published : 1998.09.01

Abstract

We cloned the 4.8 kb BglII fragment containing genes downstream pHENX7 from Pseudomonas sp. strain DJ77. The restriction map of the resultant clone, recombinant plasmid pYCS500, was determined. Sequencing analysis of the 465 bp HindIII-ClaI fragment revealed an open reading frame of 282 bp that was then designated phnM. The deduced polypeptide is 93 amino acid residues long with a $M_r$ of 10,008. The PhnM has 37.3-53.9% identity with plant-type ferredoxin proteins such as NahT, XylT, DmpQ, AtdS, PhlG, PhhQ and TbuW and contains the motif similar to well-conserved functional domains of those proteins.

Keywords

ferrodoxin;Pseudomonas sp. strain DJ77;phnM gene;reactivation

Acknowledgement

Supported by : 교육부, 환경부, 과학기술처