Screening and Identification of cellulolytic bacteria in the rumen of Korean native cattle

한우의 반추위로부터 섬유소 분해균의 탐색 및 동정

  • 김태일 (농촌진흥청 축산기술연구소) ;
  • 백순용 (성균관대학교 낙농학과) ;
  • 주이석 (농진청수의과학연구소) ;
  • 윤용덕 (농진청수의과학연구소)
  • Received : 1998.05.18
  • Accepted : 1998.07.08
  • Published : 1998.09.01

Abstract

Cellulase producing microorganisms, GPC-1, GPC-2, GNR-1 GNR-2, and GNR-3, were screened from the Rumen fluid of Korean Native Cattle. Isolated GPC-1 and GPC-2 were identified as Ruminococcus sp. according to results of the Gram stain and anaerobic characteristics. Based on morphological and physicochemical identification, the isolate GPC-1 and GPC-2 were identified as strains of Ruminococcus albus and Ruminococcus flavefaciens, respectively. Isolated GNR-1 GNR-2 and GNR-3 were identified as Bacteroides sp., Butyrivibrio sp. and Clostridium sp. according to results of the Gram stain, $H_2S$ producition and spore formation, respectively. Based on morphological and physicochemical identification, the isolate GNR-1 GNR-2 and GNR-3 were identified as strains of Bacteroides succinogenes, Butyrivibrio fibrisolvens and Clostridium cellobioparum, respectively.

Keywords

Bacteroided succinogenes;Butyrivibrio fibrisolvens;Clostridium cellobioparum;Ruminococcus albus;Ruminococcus flavefaciens