lsolation of Bacillus Strains Secreting Fibrinolytic Enzymes from Doen-Jang

된장으로부터 혈전용해능을 가진 Bacillus sp.의 분리 및 동정

  • Kim, Seung-Ho (Protein Function Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Nack-Shick (Protein Function Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Woo-Yiel (Protein Function Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jong-Woo (Chon-ju University school of Life Science) ;
  • Kim, Dong-Ho (Protein Function Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • 김승호 (생명공학 연구소 단백질기능 R.U.) ;
  • 최낙식 (생명공학 연구소 단백질기능 R.U.) ;
  • 이우일 (생명공학 연구소 단백질기능 R.U.) ;
  • 이종우 (전주대학교 생명과학부) ;
  • 김동호 (생명공학 연구소 단백질기능 R.U.)
  • Received : 1997.04.23
  • Accepted : 1998.06.12
  • Published : 1998.09.01

Abstract

Various bacterial strains that secret extracellular fibrinolytic enzyme were screened from Doen-Jang, a traditional soybean fermented food in Korea. Five microbes of them were identified to be Bacillus sp. strains according to Bergey's manual of systematic bacteriology. The culture filtrates of B. amyloliquefaciens (2.46 plasmin unit/ml) and B. pantothenticus (3.82 plasmin unit/ml) showed a level of fibrinolytic activity that was about three times higher than that of plasmin 1.0 unit and Bacillus subtilis showed the highest fibrinolytic activity (4.94 plasmin unit/ml). All of the extracellular proteases showing the fibrinolytic activity are confirmed by SDS-PAGE followed by reverse fibrin zymogram activity assay and we proposed that some of the fibrinolytic enzymes from this work are novel enzymes.

Keywords

Bacillus sp;Doen-Jang;Fibrinolytic enzyme