Metanol Metabolism and Extracellular Polysaccharide Biosynthesis in Methylovorus sp. strain SS1 DSM 11726

Methylovorus sp. strain SS1 DSM 11726의 메탄올 대사와 세포외 다당류 생합성

  • Kim, Jae. S. (Department of Biology and Bioproducts Research Center, Yonsei University) ;
  • Kim, Si W. (Division of Environmental Engineering, Chosun University) ;
  • Kim, Young M. (Department of Biology and Bioproducts Research Center, Yonsei University)
  • 김재성 (연세대학교 이과대학 생물학과, 생물산업소재연구센터) ;
  • 김시욱 (조선대학교 공과대학 환경공학부) ;
  • 김영민 (연세대학교 이과대학 생물학과, 생물산업소재연구센터)
  • Received : 1998.11.14
  • Accepted : 1998.12.16
  • Published : 1998.12.01


Melhylovorus sp. strain SS1 grown on methanol was found to show activities of key enzymes of the linear route, $NAD^+$-linked formaldehyde and formate dehydrogenases, and the cyclic route, hexulose-6-phosphate synthase, glucose-6-phosphate isomerase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase, for formaldehyde oxidation. The activities of the cyclic route enzymes were higher than those of the linear route enzymes. The bacterium also exhibited activities of the key enzymes of the ribulose monophosphate and Entner-Doudoroff pathways and transaldolase involved in the formaldehyde assimilation and the enzymes involved in the biosynthesis of extracellular polysaccharide. Cells grown in the presence of 2.3 mM ammonium sulfate were higher in the productivity of extracellular polysaccharide, but lower in the growth yield, than those grown in the presence 7.6 mM ammonium sulfate. The activities of 6-phosphogluconate dehydrogenase, phosphoglucomutase, and UDP-pyrophosphorylase in cells grown under nitrogen-limited condition were higher, but that of 6-phosphogluconate dehydratase/2-keto-3-deoxy-6-phosphogluconate aldolase was lower, than those in cells grown in the presence of sufficient amount of nitrogen source.


Methylovorus sp. SS1;methanol;methylotroph;extracellular polysaccharide


Supported by : 한국과학재단