Bacterial Diversity of the Han River as Determined by 16S rRNA Gene Analysis

16S rRNA 유전자 계통분석에 의한 한강수계의 세균 다양성

  • Han, Suk-Kyun (Dept. of Microbiology, Dankook University) ;
  • Lee, Il-Gyu (Dept. of Microbiology, Dankook University) ;
  • Ahn, Tae-Young (Research Center for Molecular Microbiology, Seoul National University)
  • 한석균 (단국대학교 자연과학대학 생명과학부) ;
  • 이일규 (단국대학교 자연과학대학 생명과학부) ;
  • 안태영 (서울대학교 분자미생물학 연구센터)
  • Received : 1998.05.07
  • Accepted : 1998.11.06
  • Published : 1998.12.01

Abstract

Bacterial diversity was determined by amplification and sequencing of 16S rDNA at Tancheon and Jungrang in Han river. Twenty-seven clones constructed were divided 7 groups using RFLP. Fifteen clones were classified 4 groups in Tancheon and the group (HT-1 clone) including many clones was affiliated a high similarity with Aerobacter cryaerophilus (the class Proteobacteria including members of the delta subdivisions). The other two groups (HT-6 and HT-9 clone) including several clones were classified with the class Cytophagales in Tancheon. Twelve clones were classified 3 groups in Jungrang and the group (HJ-1 clone) including many clones was affiliated a high similarity with Sphingomonas sp. (the class Proteobacteria including members of the alpha subdivisions). As a whole results, the class Proteobacteria (alpha, beta and delta subdivision), the order Cytophagales, and the order Actinomycetales were detected.

Keywords

16S rRNA;PCR;Phylogenetic analysis;Proteobacteria;Cytophaga

Acknowledgement

Supported by : 서울대학교 분자미생물학 연구센타, 단국대학교