Elucidation of Anti-tumor Initiator and Promoter Derived from Seaweed-3 : Anti-tumor Promoters of Ecklonia stolonifera Extracts

해조류 중의 anti-tumor initiator 및 promoter의 해석-3 : 곰피 추출물중의 발암 promotion억제 인자

  • PARK Young-Beom (Department of Food Science and Technology, Pukyong National University) ;
  • KIM In-Soo (Department of Food Science and Institute of Marine Industry, Gyeongsang National University) ;
  • YOO Sung-Jae (Department of Food Science and Technology, Pukyong National University) ;
  • AHN Jong-Khan (Department of Food Science and Technology, Pukyong National University) ;
  • LEE Tae-Gee (Department of Food Science and Technology, Pukyong National University) ;
  • PARK Douck-Chon (Department of Food Science and Technology, Pukyong National University) ;
  • KIM Seon-Bong (Department of Food Science and Technology, Pukyong National University)
  • 박영범 (부경대학교 식품공학과) ;
  • 김인수 (경상대학교 식품과학과 해양산업연구소) ;
  • 유승재 (부경대학교 식품공학과) ;
  • 안종관 (부경대학교 식품공학과) ;
  • 이태기 (부경대학교 식품공학과) ;
  • 박덕천 (부경대학교 식품공학과) ;
  • 김선봉 (부경대학교 식품공학과)
  • Published : 1998.07.01

Abstract

To elucidate anti-tumor promoter from seaweed, the anti-tumor promoting activity of Ecklonia stolonifera, Undaria pinnatifida and Laminaria japonica extracts were determined by Epstein-Barr virus (EBV)-early antigen (EA) induction caused by a tumor promoter, teleocidin B-4. The methanol extracts of seaweed were subsequently fractionated with diethyl ether, distilled water, chloroform and ethyl acetate. Among the solvent fractions tested, chloroform and ethyl acetate fraction of E. stolonifera showed a high anti-tumor promoting activity at the levels of 88.0 and $85.9\%$ by the addition of 20 ${\mu}g/m{\ell}$, respectively. To characterize anti-tumor promoters from solvent fractions of E. stolonifera, the effects of phenols, chlorophyll derivatives and carotenoids on the anti-tumor promoting activity were investigated. Phenols, such as bromophenol and phloroglucinol showed anti-tumor promoting activity of $57\~66\%$ at 20 ${\mu}g/m{\ell}$. Pigments, such as chlorophylls and carotenoids exerted high anti-tumor promoting activities. Chlorophyll a and pheophorbide a exhibited the activity of $77.4\%$ and $66.6\%$ at 5${\mu}M/m{\ell}$, respectively. The active compounds of carotenoids were tentatively identified as lutein and $\alpha-cryptoxanthin$ from the profiles of visible spectra and R_f value of their authentic compounds, and showed anti-tumor promoting activities of $76.9\%$ and $84.4\%$ at dose of 20 ${\mu}g/m{\ell}$, respectively.