The Effects of Dimethyl-Sulfoxide on the In vitro Maturation and Fertilization of Bovine Oocytes and the Subsequent Development

  • Tsuzuki, Y. ;
  • Duran, D.H. ;
  • Kuroki, Y. ;
  • Uehara, F. ;
  • Ashizawa, K. ;
  • Fujihara, N.
  • Received : 1997.11.06
  • Accepted : 1998.01.26
  • Published : 1998.06.01


The present studies were undertaken to evaluate the effects of a low concentration of dimethyl-sulfoxide (DMSO) on in vitro maturation and development of bovine oocytes fertilized in vitro. Significantly more oocytes reached the metaphase stage of the second meiotic division in TCM-199 supplemented with $50{\mu}M$ DMSO than in the control medium (p < 0.05), and the highest rates of development up to the blastocyst stage were obtained when $50{\mu}M$ DMSO was added to the maturation and culture media (p < 0.05). The avarage of cell numbers of the blastocysts, expanded and hatched blastocysts cultured with $50{\mu}M$ DMSO were 81.7, 125.7 and 129.9 cells, respectively. The proportion of blastocysts with normal chromosome numbers was 90.5%. These results suggest that the addition of $50{\mu}M$ DMSO is beneficial for the maturation of bovine oocytes and production of the blastocysts with high quality.


DMSO;IVM;IVC;Bovine Oocytes;Embryos

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