Changes of Endogenous Steroids Profile After Oral Administration of Danazol

Danazol의 경구투여에 따른 내인성 스테로이드들의 변화

  • Choi, Man Ho (Bioanalyeis & Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Chung, Bong Chul (Bioanalyeis & Biotransformation Research Center, Korea Institute of Science and Technology)
  • 최만호 (한국과학기술연구원 생체대사연구센터) ;
  • 정봉철 (한국과학기술연구원 생체대사연구센터)
  • Received : 1998.04.23
  • Published : 1998.10.25

Abstract

Danazol, one of the IOC banned substances, is a synthetic steroid which stimulates the synthesis of cellular protein with multiple and diverse biologic effects. To confirm the androgenic effects of danazol, levels of eight endogenous steroids and its major metabolite in human urine were simultaneously analyzed by selected ion monitoring (SIM) of GC/MS after oral administration. The recovery range of this method was 71.59%~93.56% and the RSD values of precision and accuracy test were 1.87%~10.48% and 1.32%~11.25%, respectively. The limits of detection of most of the drugs were $0.01{\sim}0.05{\mu}g/mL$ and calibration was carried out using urine spiked with each endogenous steroid and ethisterone standard at a concentration of 0.1, 0.5, 1.0, 10, 20 and $50{\mu}g/mL$ and with $10{\mu}g/mL$ of internal standard. Correlation coefficients varied between 0.963 and 0.991. The endogenous steroid profiling is one of valuatle tool for decteation of anabolic steroids.

Keywords

danazol;ethisterone;endogenous steroids;urinary metabolite;GC/MS