Analysis of Inorganic Acids by Capillary Zone Electrophoresis

Capillary Zone Electrophoresis를 이용한 음이온 동시분석에 관한 연구

  • Park, Sung-Woo (Department of Forensic Science, The National Institute of Scientific Investigation) ;
  • Jin, Kwang-Ho (Department of Forensic Science, The National Institute of Scientific Investigation) ;
  • You, Jae-Hoon (Department of Forensic Science, The National Institute of Scientific Investigation) ;
  • Kim, Dong-Hwan (Department of Forensic Science, The National Institute of Scientific Investigation) ;
  • Seo, Baeseuk (Department of Chemistry, Catholic University Medical College) ;
  • Kim, Young-Sang (Department of Chemistry, Korea University)
  • 박성우 (국립과학수사연구소 법과학부) ;
  • 진광호 (국립과학수사연구소 법과학부) ;
  • 유재훈 (국립과학수사연구소 법과학부) ;
  • 김동환 (국립과학수사연구소 법과학부) ;
  • 서배석 (가톨릭대학교 의과대학 화학과) ;
  • 김영상 (고려대학교 화학과)
  • Received : 1996.11.02
  • Published : 1998.06.01

Abstract

The determination of inorganic anions by capillary zone electrophoresis is reported. A ten component synthetic mixture of anions of bromide, chloride, fluoride, nitrite, nitrate, sulfite, sulfate, perchlorate, chlorate and chlorite was separated by the capillary column and detected by indirect UV method. The running buffer contained 5 mM ammonium dichromate, 10 mM ammonium acetate, 20 mM diethylenetriamine, 10% methanol solution at pH 9.3. A potential of 15 kV at the cathode (reversed polarity) was utilized for the separation of inorganic anions. A complete separation of anions was achieved in less then 10 min and the applicabilities of the method for the analysis of real samples was demonstrated. We compare the concentration of anions in toluene inhaled humen's urine and in postmortem bloods obtained by capillary zone electrophoresis and ion chromatograph.

Bromide, chloride, fluoride, nitrite, nitrate, sulfite, sulfate, perchlorate, chlorate 및 chlorite가 포함된 시료를 capillary zone electrophoresis(CZE)와 indirect UV detection 방법으로 분리하였다. 음이온류의 분리는 running buffer로 5 mM ammonium dichromate, 10 mM ammonium acetate, 20 mM diethylenetriamine, 10% methanol(pH 9.3)을 사용하였으며 전압은 cathode에 15 kV를 공급하였다. 또한 최초로 CZE에서 음이온의 정량이 가능하도록 각 이온들에 대한 regression과 detection limit를 결정하였고, 이 분석방법은 톨루엔을 흡입한 사람의 뇨, 부검 혈액, 폐수 및 폭약 등 시료에 적용하였으며, 음이온의 함량을 ion chromatograph(IC)와 비교 검토하였다.