Determination of Haloperidol Serum Levels in Psychiatric Patients with Gas Chromatography-Nitrogen Phosphorus Detection

GC/NPD를 이용한 정신분열증 환자의 혈중 Haloperidol 정량분석

  • Paik, Man-Jeong (Seoul Clinical Laboratories(SCL), Seoul Medical Science Institute) ;
  • Kang, Bo-Kyoung (Seoul Clinical Laboratories(SCL), Seoul Medical Science Institute) ;
  • Lee, Kyoung-Ok (Seoul Clinical Laboratories(SCL), Seoul Medical Science Institute) ;
  • Shin, Ho-Sang (Department of Environmental Education, Kongju University)
  • 백만정 (서울임상병리검사센터(SCL).서울과학연구소) ;
  • 강보경 (서울임상병리검사센터(SCL).서울과학연구소) ;
  • 이경옥 (서울임상병리검사센터(SCL).서울과학연구소) ;
  • 신호상 (공주대학교 환경교육학과)
  • Received : 1997.10.24
  • Published : 1998.06.01

Abstract

Analytical method of haloperidol (HAL) in serum which has been widely used in therapy of schizophrenic disorders is developed. Gas chromatography/nitrogen-phosphorus detection (GC/NPD) was used for this study. Bromoperidol was used as an internal standard and diethylether as a solvent of three-step extraction. The extraction yield in this procedure was $67.5{\pm}1.9%$ at 15 ng/mL. A good linear response in the range of 1~40 ng/mL was obtained with correlation coefficient of $r^2=0.999$. Detection limit was 0.5 ng/mL when 2 mL of serum was used. This method was applied for the analysis of HAL in serum of schizophrenic patients. After HAL decanoate (HD) was intaken as intramuscular route, HAL levels were determined at second week and forth week. From the result, the concentration of HAL at forth week appeared to 29.6% lower than those at second week. The present method showed low detection limit and high selectivity. Therefore it can be applied for the trace analysis of HAL in serum and the monitoring.