The Detection and the Antigenic Analysis of the Hepatitis G Virus in Korea

한국인에서 Hepatitis G Virus (HGV) 검출 및 항원분석에 관한 연구

  • Yoon, Jae-Deuk (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Jee, Young-Mee (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Lee, Hong-Rae (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Kim, Ki-Soon (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Kim, Young-Sun (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Lee, Yoon-Sung (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Chung, Yoon-Suk (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Park, Jeong-Koo (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Kim, Ji-Eun (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
  • Chung, Sang-In (Department of Microbiology, Medical College, Chung-Ang University) ;
  • Lee, Won-Sun (Holy Family Hospital, Bucheon City) ;
  • Lee, Won-Bae (Holy Family Hospital, Bucheon City)
  • 윤재득 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 지영미 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 이홍래 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 김기순 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 김영선 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 이윤성 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 정윤석 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 박정구 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 김지은 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 정상인 (중앙대학교 의과대학) ;
  • 이원선 (부천 성가병원) ;
  • 이원배 (부천 성가병원)
  • Published : 1998.06.30

Abstract

We investigated the rate of hepatitis G virus infection among 50 patients who were not infected with the hepatitis C virus but showed symptoms of hepatitis. Viral RNA was extracted from the patients' sera and cDNA was synthesized and amplified by RT-PCR (reverse transcription-polymerase chain reaction) using random hexamer and 5 primers (470-20-1-77F, 470-20-1-211R, 470-20-1-211R-biotin, GV57-4512MF, GV57-4657MR). The amplified PCR products were confirmed by electrochemiluminescence (ECL), liquid hybridization (LH) and Southern blotting (SB). Among the 50 PCR products, by means of ECL, we found 4 samples to be positive and 5 samples to be indeterminate. The GV45-89M probe (5'-CYCGCTGRTITGGGGTGTACfGGAAGGC-3') was end-labelled with gamma-$^{32}P$ ATP and used for liquid hybridization with the PCR products. By using liquid hybridization, we detected specific bands from 4 positive sera and also from one indeterminate serum as determined by ECL. An 1.5% agarose gel electrophoresis of the 9 PCR products which were HGV positive or indeterminate as determined by ECL showed a 160bp band from 4 positive and one indeterminate serum. The 5 PCR products proved to be positive when SB was applied with the GV45-89M probe as well as when LH was applied. LH and SB were shown to have higher sensitivity and specificity than ECL. Two cases among 5 positive cases had relatively high SGOT, SGPT, ALP values when compared with other 48 cases. In summary, we confirmed hepatitis G virus infection in 5 cases among 50 Korean patients showing symptoms of viral hepatitis.