Selection of optimum medium for mass production of Metarhizium anisopliae ARS 978

Metarhizium anisopliae ARS 978의 대량 배양을 위한 배지 조건

  • Moon, Ki-Hyuk (Dept. of Genetic Engineering, The University of Suwon) ;
  • Yoon, Jeong-Weon (Dept. of Genetic Engineering, The University of Suwon) ;
  • Yoon, Cheol-Sik (Division of Entomology, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Seung-Wook (Dept. of Chemical Engineering, Korea University)
  • 문기혁 (수원대학교 이과대학 유전공학과) ;
  • 윤정원 (수원대학교 이과대학 유전공학과) ;
  • 윤철식 (농업과학기술원 작물보호부 곤충과) ;
  • 김승욱 (고려대학교 공과대학 화학공학과)
  • Published : 1998.09.30

Abstract

The purpose of this study is to investigate the liquid culture conditions for mass production of Metarhizium anisopliae var. majus ARS 978 which is a potential microbiological pesticide. The temperature and pH range for optimal cultivation were $28^{\circ}C$ and pH 7.0, respectively. For M. anisopliae ARS 978, 1.0 %(v/v) molasses, 1.0 %(w/v) distiller's dry solubles, and 0.3 %(w/v) $CaCO_{3}$ were found to be the proper nutrients, considering cell mass, enzyme activities and spore concentration.

Keywords

Metarhizium anisopliae ARS 978;mass culture;medium establishment