Mutagenecity evaluation of insecticidal 2-carbomethoxy-4-chlorodiethyl phosphate in short-term bioassays

살충성 물질 2-carbomethoxy-4-chlorodiethyl phosphate의 유전독성 평가

  • Lee, Je-Bong (Pesticide Safety Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Sung, Ha-Jung (Pesticide Safety Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Jeong, Mi-Hye (Pesticide Safety Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kwon, Oh-Kyung (Pesticide Safety Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Hae-Keun (Pesticide Safety Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Young-Koo (Pesticide Safety Division, National Institute of Agricultural Science and Technology, RDA)
  • 이제봉 (농촌진흥청 농업과학기술원 농약안전성과) ;
  • 성하정 (농촌진흥청 농업과학기술원 농약안전성과) ;
  • 정미혜 (농촌진흥청 농업과학기술원 농약안전성과) ;
  • 권오경 (농촌진흥청 농업과학기술원 농약안전성과) ;
  • 이해근 (농촌진흥청 농업과학기술원 농약안전성과) ;
  • 김영구 (농촌진흥청 농업과학기술원 농약안전성과)
  • Published : 1998.09.30

Abstract

For evaluating the mutagenic potential of 2-carbomethoxy-4-chlorodiethyl phosphate, three different short-term mutagenicity tests were used; Salmonella typhimurium preincubation assay with and without rat liver microsomal activation, chromosome aberration test in cultured chinese hamster lung fibroblast cell and in vivo micronucleus test in male mice bone marrow. In Salmonella typhimurium reverse mutation assay using TA98, TA100, TAl535 and TAl537, 2-carbomethoxy-4-chlorodiethyl phosphate did not show any mutagenic response in the presence and absence of S9 mix. It did not induce any significant structural chromosome aberrations in the absence of metabolic activation. In micronucleus test using ICR mice, the frequency of micronucleated polychromatic erythrocytes (MNPCE) increased in bone marrow cells treated with positive control, mitomycin-C, but 2-carbomethoxy-4-chlorodiethyl phosphate did not increase micronucleated polychromatic erythrocytes. These results indicate that 2-carbomethoxy-4-chlorodiethyl phosphate does not show any positive responses in short-term genotoxicity assays.

Keywords

2-carbomethoxy-4-chlorodiethyl phosphate;mutagenicity;Ames test;chromosome aberration test;micronucleus test