Purification and Characterization of a Juvenile Hormong Binding Protein from Whole Body Homogenates of the Wax Moth, Galleris mellonella Final Instar Larvae

꿀벌부채명나방 종령유충에서 유약호르몬 결합단백질의 정제와 특성

  • Published : 1998.06.01

Abstract

A juvenile hormone binding protein (JHBP) has been isolated from the whole body homogenate of Galleria rnellonella final instar larvae by gel filtration. The isolated protein is homogenous as judged by column chromatography and gel electrophoresis in the presence and absence of denaturing agent. The JHBP has a relative molecular weight of 32 k by denaturing gel electrophresis and 28 k by gel filtration. The protein exhibits a dissociation constant of 3.9 x M for JH 111.