Antitumor Effect and the Change of Chemosensitivity of Chitosan in Human Lung Cancer Cell Line

인체 폐암세포주에 대한 키토산의 항암효과와 항암제 감수성에 미치는 영향

  • 노숙령 (중앙대학교 식품영양학과)
  • Published : 1998.05.01


This study was designed to investigated the antitumor effect and change chemosensitivity of chitosan in 2 kinds of humen lung cancer cell lines(NCI-H522, NCI-H596). To evaluate the antitumor effect and synergistic effectof chomosensitivity, MTT assay was used in vitro. then anticancer drugs used were 챤-platin , ectoposide, and adrimycin. The results of this study were as follows; Chitosan shwoed in antitumor effect on both NCI-H522 and NCI-H596. The lung cancer viability percent for NCI-H522 and NCL-H596 showed at the lowest levels of 5.31 and 5.33% when the concentration of chitosan was 25mg/$m\ell$ media and the exposure time of chitosan was 72 hours. ID50 value of chitosan on both NCI-H522 and NCI-H596 showed at the lowest levels of 14.07, 11.68 mg/$m\ell$ media when the exposure time of chitosan was 72 hours. the synergistic effect of chomosensitivity was better in NCI-H596 than in NCI0H522 . When the synergistic effect of chomosensitivity was shown according to the kind of the anticancer drugs, in case of NCI-H522 , in the concentration of 100$\mu\textrm{g}$/$m\ell$, ectoposide showed the highest synergistic effect of chomosensitivity and then was adrimycin In case of NCI-H596, in the concentration of 100$\mu\textrm{g}$/$m\ell$,, the order of the synergistic effect of chomosensitivity was ectoposide>adrimycin>cis-platin and in the concentration of 10$\mu\textrm{g}$/$m\ell$, ectoposide>cis-platin >adrimycin. It is concluded that chitosan is an active antitumor agent and is increased chomosensitivity though there is difference according to the kind and the concentration of anticancer drugs. But to be sued to lung cancer theraphy, further studies on toxicity, the mechanism of action, animal experiment are wanted.


  1. Proceeding of the second international conference on chitin/chitosan chitin and chitosan S.Suzuki;Y.Okura;K.Hashimoto;M.Suzuki;S.Hirano(ed.);S.Tokura(ed.)
  2. Food Tech v.38 no.1 Use of chitinous polymers in food Knorr,D.
  3. Science v.212 no.15 Chitin, new facets of research Austin,P.R.;Brine,S.J.;Castle,J.E.
  4. J Food Sci v.40 Reduction of suspended solids in vegetable canning waste effluents by coagulation with chitosan Bough,W.A.
  5. J Cancer v.63 Chemosensitivity testing of small cell lung cancer using the MTT assay B.G.Campling;J.Pym;H.M.Baker;S.P.C.Cole;Y.M.Lam
  6. Nutr Pept Int v.18 Hypocholesterolemic Effect of Chitosan in Cholesterol-fed rats M.Sugano;T.Fujikawa;Y.Hiratsuji;Y.Hasegawa
  7. J Food Sci v.47 Functional properties of chitin and chitosan Knorr,D.
  8. Am J Clin Nutr v.33 A novel use of chitosan as a hypocholesterolemic agent in rats M.Sugano;T.Fujikawa;Y.Hiratsuji;K.Nakashima;N.Fukuda;Y.Hasegawa
  9. 내과학회지 Suppressor genes of lung cancer Kim,Y.W.
  10. Ph.D. Thesis, Louisana State University Application of crawfish chitosan as a coagulant in recovery of organic compound from seafood processing wastes No,H.K.
  11. Biotecnology News v.2 no.4 Production and Utilization of Chitin/Chitosan Cha,W.S.;Na,J.Y.;Lee,D.B.
  12. 충남대학교 대학원 의학과 일반외과학전공 박사논문 각종 암세포주에 대한 백두옹 추출물의 항암효과 및 항암작용 백영훈
  13. J Korean Soc Food Nutr v.21 no.2 Antitumor and immunologic activity of chitosan extracted from shell of shrimp Ryu,B.H.