Effect of glucose Feeding Strategy on Biomass of Serratia marcescens in High Density Fed-Batch Fermentation

고밀도 유기식 배양에서 글루코스 공급 방법이 Serratia marcescens의 균체량에 미치는 영향

  • 김광 (동아대학교 공과대학 화학공학과) ;
  • 이상록 (동아대학교 공과대학 화학공학과) ;
  • 손정우 (동아대학교 공과대학 화학공학과) ;
  • 지홍석 (동아대학교 공과대학 화학공학과)
  • Published : 1998.12.01


Effect of glucose feeding strategy and initial concentration of glucose on Serratia marcescens ATCC 27117 in high cell density fed-batch fermentation was investigated. The final biomasses in batch, constant feeding, constant and exponentially feeding strategy at glucose starvation condition in fed-batch were 1.40, 5,07, 6,93 and 7.60 g/L at 40, 41, 24 and 40 hrs, respectively. Productivities of biomass were 0.035, 0.124, 0.289 and 0.190 g/L$.$h, respectively. As a result, constant feeding strategy at starvation condition was 1.5∼8.6 times higher than other strategies. The relationship between dissolved oxygen and glucose feeding times was good identified in exponential feeding strategy and constant feeding strategy at starvation condition. And high cell density cultivation was obtained when minimal media was used.