The Distribution of $^{14}C-chitosan$ by Different Molecular Weight in Mice

마우스에서 $^{14}C-chitosan$ 분자량별 체내 분포에 관한 연구

  • Kim, Kwang-Yoon (Department of Nuclear Medicine, Chonnam University Hospital) ;
  • Kim, Young-Ho (Department of Nuclear Medicine, Chonnam University Hospital) ;
  • Kim, Hee-Kyung (Department of Food Science and Technology, Pukyong University) ;
  • Bom, Hee-Seung (Department of Nuclear Medicine, Chonnam University Hospital) ;
  • Kim, Ji-Yeul (Department of Nuclear Medicine, Chonnam University Hospital) ;
  • Roh, Young-Bok (Department of Biology, College of Natural Sciences, Chosun University) ;
  • Nishimura, Yoshikazu (Department or Environmental Health, National Institute of Radiological Sciences)
  • Published : 1998.06.30

Abstract

Chitosan is a nontoxic natural chealtor which was made by chitin, and reduced a contamination of radiostrontium in animals. In this experiment, A different molecular weight of C-14 chitosan was intravenously administered to mice, and then the distribution of C-14 chitosan in the body was observed. Male mice (8 to 10 weeks, body weight of 30 to 35g) of ICR strain were used. C-14 chitosan was diluted with saline and then given intravenously in mice. After the administration of C-14 chitosan, mice was sacrificed at the 6th hour, 1st, 3rd, 5th, and 7th day. Beta radioactivities in the blood, liver, kidney, liver, muscle, testis, and urine was measured using a liquid scintillation analyzer. Most of the C-14 chitosan was excreted through urine within 6 hours. Biodistribution of C-14 chitosan was similar despite the difference of moleclar weight. Higher distributions of radioactivities were found in the liver, kidney, spleen. The relative concentration in tissue increased for the 6 hours and then decreased. In conclusion, most of C-14 chitosan was excreted through urine despite the difference of molecular weight. and, low molecular weight of C-14 chitosan showed higher distribution than high molecular weight of C-14 chitosan in tissues.