Multiple Age-Associated Mitochondrial DNA Deletions in Mouse Brain

  • Kim, Jin-Sun (Department of Biology, Sookmyung Women's University) ;
  • Kim, Min-Jung (Department of Biology, Sookmyung Women's University) ;
  • Kwon, In-Sook (National Institute of Agricultural Science and Technology. Division of Molecular Genetics) ;
  • Song, Eun-Sook (Department of Biology, Sookmyung Women's University)
  • Received : 1996.10.29
  • Published : 1997.01.31

Abstract

Age-dependent deletion of mitochondrial DNA (mtDNA) was detected in mouse brain using PCR method. The size of the deleted fragment was 0.5 kb, 0.9 kb. 1.7 kb and 4.3 kb in the region between cytochrome b gene and ATPase 6 gene. The deleted fragment was increased gradually from 3-month to 22month Direct repeat sequence flanking the deletion in 0.5 kb PCR product was TAAT.

References

  1. Exp. Gerontol. v.30 Ozawa, T. https://doi.org/10.1016/0531-5565(94)00057-A
  2. Am. J. Respir. Cell. Mol. Biol. v.6 Torii, K.;Sugiyama, S.;Takagi, K.;Satake, T.;Ozawa, T. https://doi.org/10.1165/ajrcmb/6.1.88
  3. Cell v.55 Wallace, D.C.;Zhang, X.;Lott, M.T.;Shoffner, J.M.;Hodge, J.A.;Kelley, R.I.;Epstein, C.M.;Hopkins, L.C. https://doi.org/10.1016/0092-8674(88)90218-8
  4. Biochem. Biophys. Res. Commun. v.164 Yuzaki, M.;Ohkoshi, N.;Kanazawa, I.;Kagawa, Y.;Ohta, S. https://doi.org/10.1016/0006-291X(89)91818-4
  5. Gene Anal. Tech. v.5 Zimmerman, E.G.;Akins, D.R.;Planz, J.V.;Schurr, M.J.
  6. Mut. Res. v.250 Ames, B.N.;Gold, L.S. https://doi.org/10.1016/0027-5107(91)90157-J
  7. Mut. Res. v.275 Arnheim, N.;Cortopassi, G. https://doi.org/10.1016/0921-8734(92)90020-P
  8. Cell v.26 Bibb, M.J.;van Etten, R.A.;Wright, C.T.;Walberg, M.W.;Clayton, D.A. https://doi.org/10.1016/0092-8674(81)90300-7
  9. Aging Clin. Exp. Res. v.6 Chung, S.S.;Weindruch, R.;Schwarze, S.R.;McKenzie, D.I.;Aiken, J.M. https://doi.org/10.1007/BF03324239
  10. Nature Genetics v.2 Corral-Debranski, M. https://doi.org/10.1038/ng1292-324
  11. Mut. Res. v.275 Corral-Debranski, M.;Shoffner, J.M.;Lott, M.T.;Wallace, D.C. https://doi.org/10.1016/0921-8734(92)90021-G
  12. Nucleic Acids Res. v.18 Cortopassi, G.A.;Arnheim, N. https://doi.org/10.1093/nar/18.23.6927
  13. J. Am. Geriatr. Soc. v.20 Harman, D. https://doi.org/10.1111/j.1532-5415.1972.tb00787.x
  14. Biochem. Biophys. Res. Commun v.170 Ikebe, S. https://doi.org/10.1016/0006-291X(90)90497-B
  15. Naturwissenschaften v.77 Kadenbach, B. https://doi.org/10.1007/BF01138485
  16. Mut. Res. v.275 Linnane, A.W.;Zhang, C.;Baumer, A.;Nagley, P. https://doi.org/10.1016/0921-8734(92)90023-I
  17. Ann. Neurol. v.34 Mecocci, P.;Macgarvey, U.;Kaufman, A.E.;Koontz, D.;Shoffner, J.M.;Wallace, D.C.;Beal, M.F. https://doi.org/10.1002/ana.410340416
  18. Nucleic Acids Res. v.18 Mita, S.;Rizzuto, R.;Moraes, C.T.;Shanske, S.;Arnaudo, E.;Fabrizi, G.M.;Koga, Y.;DiMauro, S.;Schon, E.A. https://doi.org/10.1093/nar/18.3.561