Optimal Culture Conditions for Doxorubicin Production by a Mutant of Streptomyces peucetius subsp. caesius

Streptomyces peucetius subsp. caesius 돌연변이주에 의한 doxorubicin생산의 최적배양조건

  • Kim, Seung-Wook (Department of Chemical Engineering, Korea University) ;
  • Song, Soo-Moon (Department of Genetic Engineering, The University of Suwon) ;
  • Moon, Soon-Ok (Department of Genetic Engineering, The University of Suwon)
  • Received : 1997.04.22
  • Accepted : 1997.06.05
  • Published : 1997.08.10


The production of doxorubicin by a mutant of Streptomyces peucetius subsp. caesius was studied. The optimal culture conditions, such as inoculum size and medium composition were established to improve the productivity of doxorubicin. The optimal medium composition was found to be 4% maltose, 0.5% HEPES, 0.02% $K_2HPO_4$, 0.01% $MgSO_4$. As an antiform agent, 0.001% KG(10% Adekanol+10% Silicone) was suitable one among various agents. Culture was carried out in 2.5 L jar-fermenter with different aeration rates of 1.5, 1.0, and 1.5 v/v min. The maximum production of doxorubicin(29 mg/l) was obtained at 1.5 v/v min of aeration rate, and even at 1.0 v/v min, the production of doxorubicin was increased up to 15% compared with that of shake-flask culture.



Supported by : 교육부


  1. Biotechnology of Industrial Antibiotics E. J. Vandamme
  2. Biotechnology v.4 H. J. Rhem;G. Reed
  3. Biotechnol. Bioeng. v.11 F. Arcamone;G. Cassinelli;G. Frantini;A. Grein;P. Orezzi;C. Pal;C. Spall
  4. Mol. Pharmacol. v.41 N. R. Bachur;F. Yu;R. Johnson;R. Wickey;Y. Wu;L. Markas
  5. J. Antibiot. v.33 A. Yoshimoto;T. Oki;H. Umezawa
  6. J. Antibiot. v.34 T. Oki;Y. Takatsuki;H. Tobe;A. Yoshimoto;T. Takeuchi;H. Umezawa
  7. Can. J. Microbiol. v.31 M. L. Dekleva;J. A. Titus;W. R. Strohl
  8. J. Can. Microbiol. v.33 M. L. Dekleva;W. R. Strobl
  9. Process Biochem. v.14 J. C. McGuire;B. K. Hamilton;R. J. White
  10. Medical Series F. Arcamone
  11. Microbiol. Reviews v.44 J. F. Martin;A. L. Demain
  12. Cold Spring Harbour Laboratory K. F. Chater
  13. Basic biotechnolgy J. Bulock;B. Kristiansen
  14. U.S. patent No. 4,592,999 H. Umezawa;K. Kouno;T. Ishihara;A. Yoshimoto;Y. Takatsuki;H. Tobe
  15. Appl. Microbiol. Biotechnol. v.26 A. Lebrihi;P. Germain
  16. U.S. patent No. 3997662 S. Pinnert;J. Preud' Homme