Development of New Separation Technique, Modifier Composition Programming in Supercritical Fluid Chromatography

초임계 유체 크로마토그래피에서 새로운 분리방식인 변형제 조성 프로그래밍법 개발

  • Kim, Hohyun (Department of Chemistry, Kangwon National University) ;
  • Pyo, Dongjin (Department of Chemistry, Kangwon National University)
  • 김호현 (강원대학교 자연과학대학 화학과) ;
  • 표동진 (강원대학교 자연과학대학 화학과)
  • Received : 1997.05.10
  • Published : 1997.10.25


Supercritical Fluid Chromatography(SFC) has been developed as an analytical technique for the compounds that is difficult to analyze by conventional chromatography. Since supercritical fluid $CO_2$ is difficult to elute solutes with high polarity, modified supercritical $CO_2$, was used as a mobile phase. In conventional method, silica column which is saturated with modifier was used. However, with this method, we can not control the quantity of modifier. In this paper, we developed a new method which can control quantity of modifier mixed in supercritical fluid $CO_2$. The quantity of $H_2O$ mixed was measured with amperometric microsensor which was made by perflurosulfonate ionomer(PFSI) film. we have also obtained a good supercritical fluid chromatogram of PAH mixture by use of a modifier composition programming method.


supercritical fluid chromatography;modifier programming


Supported by : 한국학술진흥재단