Analysis of Kasugamycin in Pesticide Formulations by Reversed-Phase Ion Pair Liquid Chromatography

역상 이온쌍 액체크로마토그래피에 의한 농약 제품 중 Kasugamycin의 분석

  • Kim, Taek-Jae (Department of Chemistry, Kyonggi University) ;
  • Kim, Kyong-Sun (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology) ;
  • Yoon, Chae-Hyuk (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology) ;
  • Joo, Jin-Bok (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology) ;
  • Kim, Chung-Hyo (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology)
  • 김택제 (경기대학교 이과대학 화학과) ;
  • 김경선 (농업과학기술원 작물보호부 농약품질과) ;
  • 윤채혁 (농업과학기술원 작물보호부 농약품질과) ;
  • 주진복 (농업과학기술원 작물보호부 농약품질과) ;
  • 김충효 (농업과학기술원 작물보호부 농약품질과)
  • Received : 1997.04.10
  • Published : 1997.10.25

Abstract

Analytical method using the reversed phase ion-pair chromatography (RP-IPC) for the determination of kasugamycin(5-amino-2-methyl-6-(2,3,4,5,6-pentahydroxy cyclohexyloxy)tetrahydropyran-3-yl-amino-${\alpha}$-imino acetic acid), pesticide as fungicide bactercide has been established. The retention behavior of kasugamycin in the RP-IPC was examined with respect to the effect of concentrations of organic modifiers, pH of eluent and types and concentrations of the counter ions as ion-pair reagent. This method developed by the optimum factors, can be used for the application of the quality control in the crude product and its formulation.

Keywords

reversed phase;ion-pair;HPLC;kasugamycin