A Study on the Analysis of Polycyclic Aromatic Hydrocarbons by RPLC/DAD (I)

RPLC/DAD를 이용한 Polycyclic Aromatic Hydrocarbon류의 분석에 관한 연구(I)

  • Lee, Won (Research Instituted for Basic Science and Department of Chemistry, Kyunghee University) ;
  • Hong, Jee-Eun (Doping Control Center, Korea Institute of Science and Technology) ;
  • Park, Song-Ja (Doping Control Center, Korea Institute of Science and Technology) ;
  • Pyo, Hee Soo (Doping Control Center, Korea Institute of Science and Technology)
  • 이원 (경희대학교 기초과학연구소, 문리과학대학 화학과) ;
  • 홍지은 (한국과학기술연구원 도핑콘트롤센터) ;
  • 박송자 (한국과학기술연구원 도핑콘트롤센터) ;
  • 표희수 (한국과학기술연구원 도핑콘트롤센터)
  • Received : 1997.03.28
  • Published : 1997.10.25

Abstract

The retention behaviors of 16 PAHs and 4 nitro-PAHs were studied with several parameters involved numbers of carbon atoms, F factor, aqueous solubility, L/B ratio, and numbers of interfering hydrogen atom pairs on the chemical structures of PAHs by using reversed-phase liquid chromatography/diode array detection method (RPLC/DAD) and gradient elution method. It was obtain that the log k' for most of PAHs with increasing the number of carbon and the F factor in their molecules. Chromatographic retention of PAH isomers and nitro-PAHs were examined with aqueous solubility, L/B ratio and number of interfering hydrogen atom pairs. As a result of comparison with these factors and retention times, it was found that those solutes having larger aqueous solubilities and greater L/B ratios were retained longer on stationary phase. This tendency was also occured in the molecules having the more number of interfering hydrogen atom pairs. Detection limits of PAHs which were obtained with three times measurements by RPLC/DAD were in the range of 100~500ng/mL and method detection limit(MDL) for water sample were in the range of 0.1~0.5ng/mL.

Keywords

polycyclic aromatic hydrocarbons;RPLC/DAD;retention behavior

Acknowledgement

Supported by : 교육부 기초과학연구소