Development of New Analytical Method of Vitamins Using Supercritical Fluid

초임계 유체를 이용한 비타민류의 새로운 분석법 개발

  • Pyo, Dongjin (Department of Chemistry, Kangwon National University) ;
  • Park, Dongjin (Department of Chemistry, Kangwon National University) ;
  • Kim, Hohyun (Department of Chemistry, Kangwon National University) ;
  • Lee, Hakju (Department of Chemistry, Kangwon National University) ;
  • Lee, Taejoon (Department of Chemistry, Kangwon National University)
  • 표동진 (강원대학교 자연과학대학 화학과) ;
  • 박동진 (강원대학교 자연과학대학 화학과) ;
  • 김호현 (강원대학교 자연과학대학 화학과) ;
  • 이학주 (강원대학교 자연과학대학 화학과) ;
  • 이태준 (강원대학교 자연과학대학 화학과)
  • Received : 1996.11.18
  • Published : 1997.04.25

Abstract

Supercritical Fluid Chromatography(SFC) has become a technique for solving problems that are difficult to be monitored by other chromatographic methods. However, the most widely used fluid, is no more polar than hexane. Polar samples which are difficult to be analyzed with pure supercritical $CO_2$ because of their high polarity can be separated by adding polar modifiers to supercritical $CO_2$. In this paper, a new method for monitoring the mobile phase composition in modified supercritical fluid chromatography was developed. The amount of water dissolved in supercritical $CO_2$ was measured by amperometric microsensor which is made of thin film of perfluorosulfonate ionomer(PFSI). The amount of water dissolved in supercritical $CO_2$ stayed constant for a much longer time than with a saturator column. With this new mixing device, we could get good resolutions for vitamins which are difficult to separate with pure $CO_2$.

Acknowledgement

Supported by : 대송교역, 산학협동재단