A Study on the Extraction Efficiency of Berberine in Coptidis Rhizoma

황련 중 berberine의 추출효율에 관한 연구

  • Rhee, Jae Seong (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Park, Ho Koon (Department of Applied Science, Korea Institute of Science and Technology) ;
  • Woo, Eun Ran (Department of Applied Science, Korea Institute of Science and Technology) ;
  • Lee, Eun Ju (Department of Oriental Medicine, Kyung Hee University) ;
  • Ahn, Dug Kyun (Department of Oriental Medicine, Kyung Hee University) ;
  • Youn, Won Sik (Department of Oriental Medicine, Kyung Hee University) ;
  • Shin, Kwang Ho (Department of Oriental Medicine, Kyung Hee University)
  • 이재성 (한구과학기술연구원 특성분석센터) ;
  • 박호군 (한국과학기술연구원 응용과학부) ;
  • 우은란 (한국과학기술연구원 응용과학부) ;
  • 이은주 (경희대학교 본초학교실) ;
  • 안덕균 (경희대학교 본초학교실) ;
  • 윤원식 (경희대학교 본초학교실) ;
  • 신광호 (경희대학교 본초학교실)
  • Received : 1996.07.26
  • Published : 1997.04.25

Abstract

In this paper, optimum extraction condition for berberine which is the indan companent of Coptidis rhizoma was examined at various conditions in the aspect of temperature, solvent and time followed by quantitation with reversed phase HPLC. The efficiency for extraction has been shown the best at $60^{\circ}C$ with 2~3 hours reflux time in methanol or acetic acid. Any significant effect can not be shown from the addition of SLS to extraction solvent.