Isolation and Characterization of a Bioemulsifier-Producing Bacterium for Marine Oil Spill Bioremediation

해양유류오염 방제를 위한 생물유화제 생산세균의 분리 및 특성

  • 손홍주 (부산대학교 환경문제연구소) ;
  • 차미선 (부산대학교 환경문제연구소, 부산대학교 미생물학과)
  • Published : 1997.10.01

Abstract

Microorganisms producing bioemulslfiler were isolated from the sea water In Pusan coastal area. The isolated strain which had the highest emulsification activity and stability was identified as the genus Achetobacter from the results of morphological. cultural and biochemical tests and named Achetobacter sp. EL-C6 for convenience. The compositions of optimum medium for emulsification of crude oil by Acinetobacter sp. EL-C6 were crude oil 2.0%, NH4NO3 0.2%, $K_2HPO_4$ 0.01%, $MgSO_4$.$7H_2O$ 1.o%, $CaCl_2$.$2H_2O$ 0.1% and NaCl 3.0% at initial pH 7.5 and 3$0^{\circ}C$, respectively. The cultivation for emulsification of crude ell was carried out in 500m1 shaking flask containing 100m1 of the optimum medium at 3$0^{\circ}C$. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Achetobacter sp. EL-C6 showed that utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, crude ell was best utilized by the Achetobacter sp. EL-C6.

Keywords

Acinetobacter sp. EL-C6;crude oil;emulsification

References

  1. J Am. Oil Chem. Soc. v.46 Surfactants for oil recovery Ahearn, G.P.
  2. Bergey's Manual of Determinative Bacteriology(8th ed.) Krieg, N.R.;J.G. Holt
  3. Advan. Biochem. Engineer, Biotechnol. v.33 Extracellular microbial mipids as biosurfactants Haferburg, D.;R. Hommel
  4. Annu. Rev. Microbiol. v.31 Oil tankers and pollution : a microbiological approach Gutnick, D.L.;E. Rosenberg
  5. Biotech. Advs. v.3 Biosurfactants Parkinson, M.
  6. J. Mar. Biol. v.48 Toxicity of oil spill removers(detergent) to marine life Corner, E.D.S.;A.J. Southward;E.C. Southward
  7. Mar. PolBull. v.3 Microbial degradation of oil Floodgate, G.D.
  8. Kor. J. Microbiol. v.30 no.3 경기만에서 석유분해 세균이 분포 및 석유분해능 이정대;황열순;이기순;이건영;김상종
  9. Appl. Environ. Microbiol. v.60 Survival of lux-lac-marked biosurfactant-producing Pseudomonas aeruginosa UG2L in soil monitored by nonselective plating and PCR Flemming, C.A.;K.T. Leung;H. Lee;J.T. Trevors;C.W. Greer
  10. Manual of Methods for General Bacteriology Gerhardt, P.;R.G.E. Murray;R.N. Costilow;E.W. Wester
  11. Appl. Microbiol. v.23 Microbial utilization of crude oil Jobson A.;F.D. Cook;D.W.S. Westlake
  12. Bergey's Manual of Systematic Bacteriology v.2 Krieg, N.R.;J.G. Holt
  13. The Prokaryotes v.2 Starr, M.P.;N. Stlop, H.G.;Truper, A. Balows;H.G. Schleger
  14. 시험연구보 한국의 해양오염 현황과 대책 양 경찰대
  15. Kor. J. Microbiol. Bioeng. v.16 no.5 해양세균 Achromobacter sp. M-1220 균주에 의한 bunker-C oil의 유화 박중연;박인식;서근학;홍용기
  16. Biochemical Tests for Identification of Medical Bacteria Macfaddin, J.F.
  17. Oil Gs. J. v.46 Bacterial release of oil from sedimentary materials Zobell, C. E.
  18. Kor. J. Appl. Microbiol. Bioeng. v.16 no.5 Emulsiflcation of bunker-C oil by a marine bacterium Achromobacter sp. M-1220 Park, J.Y.;I.S. Park
  19. The microbial degradation of oil pollutants Effect of some commercial oil herders dispersants and bacterical inoculum on biodegradation of oil in sea water Atlas, R.M.;R. Bartha;D.C. Ahearn(ed);S.P. Meyer(ed)
  20. Bergey's Manual of Determinative Bacteriology(8th ed.) Krieg, N.R.;J.G. Holt
  21. Microbiological Review v.45 no.1 Microbial degradation of petroleum hydrocarbon : an environmental perspective Atlas, R.M.