Isolation and Characterization of a Bioemulsifier-Producing Bacterium for Marine Oil Spill Bioremediation

해양유류오염 방제를 위한 생물유화제 생산세균의 분리 및 특성

  • 손홍주 (부산대학교 환경문제연구소) ;
  • 차미선 (부산대학교 환경문제연구소, 부산대학교 미생물학과)
  • Published : 1997.10.01


Microorganisms producing bioemulslfiler were isolated from the sea water In Pusan coastal area. The isolated strain which had the highest emulsification activity and stability was identified as the genus Achetobacter from the results of morphological. cultural and biochemical tests and named Achetobacter sp. EL-C6 for convenience. The compositions of optimum medium for emulsification of crude oil by Acinetobacter sp. EL-C6 were crude oil 2.0%, NH4NO3 0.2%, $K_2HPO_4$ 0.01%, $MgSO_4$.$7H_2O$ 1.o%, $CaCl_2$.$2H_2O$ 0.1% and NaCl 3.0% at initial pH 7.5 and 3$0^{\circ}C$, respectively. The cultivation for emulsification of crude ell was carried out in 500m1 shaking flask containing 100m1 of the optimum medium at 3$0^{\circ}C$. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Achetobacter sp. EL-C6 showed that utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, crude ell was best utilized by the Achetobacter sp. EL-C6.


Acinetobacter sp. EL-C6;crude oil;emulsification


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