Isolation of Non-toxic Bacillus thuringiensis Strains from the Dead Larvae of Apriona germari and Aphodius apicalis

뽕나무하늘소(Apriona germari) 및 왕똥풍뎅이 (Aphodius apicalis) 사충으로부터 무독성 Bacillus thuringiensis의 분리

  • 장진희 (서울대학교 응용생물화학부 및 농업생물신소재연구센터) ;
  • 박현우 (서울대학교 응용생물화학부 및 농업생물신소재연구센터) ;
  • 진병래 (서울대학교 응용생물화학부 및 농업생물신소재연구센터) ;
  • 윤형주 (농촌진흥청 잠사곤충연구소) ;
  • 마형일 (농촌진흥청 잠사곤충연구소) ;
  • 강석권 (서울대학교 응용생물화학부 및 농업생물신소재연구센터)
  • Published : 1997.09.01

Abstract

Four strains of Bacillus thuringiensis were isolated froin the dead larvae of mulberry longicorn beetle (Apriong germari) and dung beetle (Aphodius apicalis). One nf four B. thuringiensis isolates turned out to be subspecies darinstadiensis but the remains were not identified using 33 B. thuringiensts flgellar ( H ) antibodies. Furthermore. bioassays of spore-parasporal inclusion protein mixture conducted against third instar larvae of A. gerrntrri or A. apicalis, second instar larvae of Bombyx mori, and third instar larvae of Cu1ex pipiens pullens showed that the isolates were non-toxic. To further confirm, four isolates were characterized and analysed by SDS-PAGE and agarose gel electrophoresis. The results revealed that parasporal protein and plasmid DNA patterns of four isolates are different from those of darmstadiensis and 20 known non-toxic B. thuringiensis strains, suggesting that the four isolates are novel non-toxic B. thuringiensis.