Physiological factors affecting rapid cold hardening of the beet armyworm, Spodoptera exigua(Hubner)

파밤나방(Spodoptera exigua(Hubner))의 내한성유기 관련인자 분석

  • 송원례 (안동대학교 자연과학대학 생명자원과학부 곤충생리실) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학부 곤충생리실) ;
  • 조점래 (농업과학기술원 작물보호부 곤충과) ;
  • 김홍선 (농업과학기술원 작물보호부 곤충과) ;
  • 이정운 (농업과학기술원 작물보호부 곤충과)
  • Published : 1997.09.01

Abstract

The sublethal temperature (5$^{\circ}$C for 2hr) led the fifth instar larvae of beet armyworm, Spodoprern exigucr (Hiibner), to increase cold tolerance to subzero lethal temperatures ( 'rapid cold hardening' ). The strength of rapid cold hardening was, however, varied among different populations which showed different cold tolerance in response to cold temperatures. To analyse the physiological factors affecting the rapid cold hardening, hernolymph osmolalities. supercooling points, glycerol contents, and cold stress proteins were measured by treating the fifth instar larvae with the sublethal low temperatures. The treated larvae showed increase of hemolymph osmolalities and glycerol contents. Changes of the osmolalities were greater in cold-hardy strains than in cold-susceptible strains. The sublethal temperature also induced them to express the cold-stress proteins (I0 - 20kD) in the hemolymph. but did not to change supercooling points.