Helicobacter pylori 특이 계란항체의 생산 및 특성

  • Kim, Byoun-Jae (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kang, Byung-Hwa (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kim, Tae-Yong (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kim, Tae-Han (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kim, Kee-Won (Research Laboratories, Il-dong Pharmaceutical Co.)
  • 김병재 (일동제약(주) 중앙 연구소) ;
  • 강병화 (일동제약(주) 중앙 연구소) ;
  • 김태용 (일동제약(주) 중앙 연구소) ;
  • 김태한 (일동제약(주) 중앙 연구소) ;
  • 김기원 (일동제약(주) 중앙 연구소)
  • Published : 1997.12.01

Abstract

IgY (egg yolk immunoglobulin) against Helicobacter pylori was produced by immunizing hen with some Helicobacter pylori antigens. H. pylori whole cell, whole cell lysates, partially purified urease and p54 protein, which showed high antigenicity in mice, were used as immunogens. Four hens were immunized with these immunogens three times. IgY was purified from immunized egg yolk with polyethylene glycol (M.W. 8000) and its anti-H. pylori titer was determined by enzyme linked immunosorbent assay (ELISA). The anti-H. pylori titer reached peak at 8 weeks and was maintained over 20 weeks. H. pylori cells were agglutinated with these purified IgY and the specificity of these purified IgY was detected with immunoblotting.

Keywords

lgY;Chicken immunoglobulin;Purification;Helicobacter pylori;Urease;p54;Agglutination;ELISA

References

  1. J. Vet. Med. v.A38 Distribution of immunoglobulins during embryogenesis in the chicken Kaspers,B.;I.Schranner;U.Losch
  2. J. Food. Sci. v.53 Anti-E. coli immunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient Shimizu,M.;R.C.Fitzsimmos;S.Nakai
  3. Proc. Natl. Acad. Sci. v.76 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications Towbin,H.;T.Staehelin;J.Gordon
  4. J. Immunol. Methods v.160 Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain Akita,E.M.;S.Nakai
  5. Infect. Immun. v.60 Identification and purification of a cpn60 heat shock protein homolog from Helicobacter pylori Dunn,B.E.;R.M.RoopⅡ;C.C.Sung;S.A.Sharma;G.I.Perez-Perez;M.J.Blaser
  6. Agric. Biol. Chem. v.54 A novel isolation method for hen egg yolk antibody, IgY Hatta,H.;M.Kim;T.Yamamoto
  7. Infect. Immun. v.64 Antigen recognition during progression from acute to chronic infection with a cagA-positive strain of Helicobacter pylori Hazel,M.M.;S.L.Hazell;T.Kolesnikow;J.Mitchell;D.Frommer
  8. Lancet v.i Unidentified curved bacilli on gastric epithelium in active chronic gastritis Warren,J.R.;B.Marshall
  9. J. Infect. Diseases v.153 Campylobacter pyloridis and gastritis:Association with intercellular spaces and adaptation to an environment of mucus as important factors in colonization of the gastric epithelium Hazell,S.L.;A.Lee;L.Brady;W.Hennessy
  10. Infect. Immun. v.64 Effect of Helicobacter pylori on gastric epithelial cell migration and proliferation in vitro: Role of VacA and CagA Ricci,V.;C.Ciacci;R.Zarrilli;P.Sommi;M.K.Tummuru;C.D.V.Blanco;C.B.Bruni;T.L.Cover;M.J.Blaser;M.Romano
  11. Can. Inst. Food. Sci. Technol. J. v.22 Isolation of egg yolk immunoglobulin-rich fractions using copper-loaded metal chelate interaction chromatography McCannel,A.;S.Nakai
  12. Gastroenterology v.109 Oral immunization with Helicobacter pylori urease B subunit as a treatment against Helicobacter infection in mice Theulaz,I.C.;N.Porta;M.Glauser;E.Saraga;A.C.Vaney;R.Haas;J.P.Kraehenbuhl;A.L.Blum;P.Michetti
  13. Infect. Immun. v.60 Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets Yokoyama,H.;R.C.Peralta;R.Diaz;S.Sendo;Y.Ikemori;Y.Kodama
  14. Handbook of microbiological media Urea broth base Atlas,R.M.;L.C.Parks(ed.)
  15. New Eng. J. Med. v.324 Helicobacter pylori and peptic ulcer disease Peterson,W.L.
  16. Infect. Immun. v.61 Purification of Helicobacter pylori superoxide dimutase and cloning and sequencing of the gene Spiegelhalder,C.;B.Gerstenecker;A.Kersten;E.Schiltz;M.Kist
  17. J. Infect. Diseases v.142 Prevention of murine rotavirus infection with chicken egg yolk immunoglobulins Bartz,C.R.;R.H.Conklin;C.B.Tunstall;J.H.Steele
  18. Antimicrob. Agents Chemotherapy v.39 Bactericidal activity of a new antiulcer agent, Ecabet Sodium, against Helicobacter pylori under acidic conditions Shibata,K.;Y.Ito;A.Hongo;A.Yasoshima;T.Endo;M.Ohashi