Helicobacter pylori 특이 계란항체의 생산 및 특성

  • Kim, Byoun-Jae (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kang, Byung-Hwa (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kim, Tae-Yong (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kim, Tae-Han (Research Laboratories, Il-dong Pharmaceutical Co.) ;
  • Kim, Kee-Won (Research Laboratories, Il-dong Pharmaceutical Co.)
  • 김병재 (일동제약(주) 중앙 연구소) ;
  • 강병화 (일동제약(주) 중앙 연구소) ;
  • 김태용 (일동제약(주) 중앙 연구소) ;
  • 김태한 (일동제약(주) 중앙 연구소) ;
  • 김기원 (일동제약(주) 중앙 연구소)
  • Published : 1997.12.01


IgY (egg yolk immunoglobulin) against Helicobacter pylori was produced by immunizing hen with some Helicobacter pylori antigens. H. pylori whole cell, whole cell lysates, partially purified urease and p54 protein, which showed high antigenicity in mice, were used as immunogens. Four hens were immunized with these immunogens three times. IgY was purified from immunized egg yolk with polyethylene glycol (M.W. 8000) and its anti-H. pylori titer was determined by enzyme linked immunosorbent assay (ELISA). The anti-H. pylori titer reached peak at 8 weeks and was maintained over 20 weeks. H. pylori cells were agglutinated with these purified IgY and the specificity of these purified IgY was detected with immunoblotting.


lgY;Chicken immunoglobulin;Purification;Helicobacter pylori;Urease;p54;Agglutination;ELISA


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