Baeuveria sp. C208의 대량 배양을 위한 생산배지의 최적화

  • Moon, Ki-Hyuk (Department of Genetic Engineering, The University of Suwon) ;
  • Kim, Pyong-Hyok (Department of Chemical Engineering, Korea University) ;
  • Yoon, Jeong-Weon (Department of Genetic Engineering, The University of Suwon) ;
  • Sung, Jae-Mo (Department of Agricultural Biology, Kangwon National University) ;
  • Kim, Seung-Wook (Department of Chemical Engineering, Korea University)
  • 문기혁 (수원대학교 유전공학과) ;
  • 김병혁 (고려대학교 화학공학과) ;
  • 윤정원 (수원대학교 유전공학과) ;
  • 성재모 (강원대학교 농생물학과) ;
  • 김승욱 (고려대학교 화학공학과)
  • Published : 1997.12.01

Abstract

Entomogenous fungi which attack living insects are powerful means for microbiological insecticide. The purpose of this study is to establish the culture conditions and media for mass production of Beauveria sp. C208 which has a broad host range as a potential microbiological pesticide. The temperature and pH range for optimal cultivation of this strain were 28$circ$C and pH 5.0-7.0. For Beauveria sp. C 208, 2% rice straw and 0.6% tryptone were found as the proper carbon and nitrogen sources, considering cell mass, enzyme activities such as chitinase, protease and lipase, and spore concentration.

Keywords

Beauveria sp. C208;Mass culture;Medium Optimization

References

  1. Kor. J. Appl. Microbiol. Biotechnol. v.18 Transfer of insecticidal toxin gene in plant: cloning of insecticidal protein gene in Bacillus thuringiensis Lee,H.H.;S.H.Hwang;Y.S.Park
  2. J. Invertebr. Pathol. v.64 Relationships among enzyme activities and virulence parameters in Beauveria bassiana interactions of Galleria mellonella and Trichoplusia ni. Gupta,S.C.;T.D.Leathers;G.N.Er-Sayed;C.M.Ignoffo
  3. Biocontrol Sci. Technol. v.4 Production, formation and application of the entomopathogenic fungus Beauveria bassiana for insect control:current status Feng,M.G.;T.J.Poprawski;G.G.Khachatourians
  4. Biology of Conidial Fungi v.2 Entomogenous fungi Roberts,D.W.;R.A.Humber;G.T.Cole(ed.);B.Kendrick(ed.)
  5. J. Invertebr. Pathol. v.61 Induction and purification of Beauveria bassiana chitinolytic enzyme Havukkala,I.;C.Mitamura;S.Hara;K.Hirayae;Y.Nishizawa;T.Hibi
  6. Annu. Rev. Entomol v.39 Interaction between fungal pathogens and insect hosts Hajek,A.E.;R.J.St.Leger
  7. Exp. Mycol. v.16 Insect cuticle-degrading enzymes from the entomogenous fungus Beauveria bassiana Gupta,S.C.;T.D.Leathers;G.N.Er-Sayed;C.M.Ignoffo
  8. Biotechnology v.3 Microbial insecticides Quinlan,R.J.;S.G.Lisansky;H.J.Rehm(ed.);G.Reed(ed.);H.Dellweg(ed.)
  9. Biocontrol News and Inform. v.14 The potential of mycoinsecticides Moore,D.;C.Prior.
  10. Kor. J. Appl. Microbiol. Biotechnol. v.21 Growth and production of endotoxin of Bacillus thuringiensis isolates Lee,J.Y.;G.J.Park;H.H.Lee
  11. Enzyme Microb. Technol. v.14 Formation of extracellular lipases by filamentous fungi, yeasts, and bacteria Rapp,P.;S.Backhaus