곰팡이 분리주 MT60109가 생산하는 Phospholipase C 저해물질의 분리

  • Oh, Won-Keun (Cellular Response Modifier Research Unit., Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Hyun-Sun (Cellular Response Modifier Research Unit., Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Chan-Sun (Cellular Response Modifier Research Unit., Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Soon-Cheol (Cellular Response Modifier Research Unit., Korea Research Institute of Bioscience and Biotechnology) ;
  • Ko, Hack-Ryong (Cellular Response Modifier Research Unit., Korea Research Institute of Bioscience and Biotechnology) ;
  • Mheen, Tae-Ick (Cellular Response Modifier Research Unit., Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Jong-Seog (Cellular Response Modifier Research Unit., Korea Research Institute of Bioscience and Biotechnology)
  • 오원근 (생명공학연구소, 세포반응조절 연구Unit) ;
  • 이현선 (생명공학연구소, 세포반응조절 연구Unit) ;
  • 박찬선 (생명공학연구소, 세포반응조절 연구Unit) ;
  • 안순철 (생명공학연구소, 세포반응조절 연구Unit) ;
  • 고학룡 (생명공학연구소, 세포반응조절 연구Unit) ;
  • 민태익 (생명공학연구소, 세포반응조절 연구Unit) ;
  • 안종석 (생명공학연구소, 세포반응조절 연구Unit)
  • Published : 1997.12.01

Abstract

During the screening of inhibitors against phospholipase C (PLC) and the formation of inositol phosphates (IP$_{t}$) at NIH3T3${\gamma}$1 cells from microbial secondary metabolites, we selected a fungal strain MT60109 which was capable of producing an inhibitor. By the taxonomic studies, this fungus was identified as Pseudallescheria sp. MT60109 and an inhibitor of PLC was purified by BuOH extraction and chromatographic techniques from the culture broth of Pseudallescheria sp. MT60109. The inhibitor was identified as thielavin B by the physico-chemical properties and spectroscopic analysis of UV, FAB-MS, $^{1}$H, $^{13}$C-NMR, $^{1}$H-$^{1}$H COSY and HMBC. Thielavin B showed potent inhibitory activity against PLC purified from bovine brain with an IC$_{50}$ of 20 $\mu$M. And it also inhibited the formation of inositol phosphates in platelet-derived growth factor (PDGF) -stimulated NIH3T3${\gamma}$1 cells with an IC$_{50}$ of 20 $\mu$M.

Keywords

Phospholipase C;Inositol phosphates formation;Fungi isolate;Thielavin B.

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