Streptomyces sp. 50634 균주가 생산하는 tipA Promotor 활성화 물질, Sulfomycin Ia

  • Shim, Yong-Ho (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yun, Bong-Sik (Korea Research Institute of Bioscience and Biotechnology) ;
  • Seto, Haruo (Institute of Molecular and Cellular Biosciences, The University of Tokyo) ;
  • Hwang, Se-Young (Graduate School of Biotechnology, Korea University) ;
  • Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology)
  • 심용호 (생명공학연구소) ;
  • 윤봉식 (생명공학연구소) ;
  • 세또 하루오 (동경대학 분자세포생물학연구소) ;
  • 황세영 (고려대학교 생명공학원) ;
  • 유익동 (생명공학연구소)
  • Published : 1997.12.01

Abstract

In the course of screening for the tipA promoter-inducing substances, we isolated an active compound, sulfomycin Ia, from the mycelium of a microorganism designated 50634. The producing organism was identified as Streptomyces sp. on the basis of taxonomic studies. Sulfomycin Ia was purified from mycelial extract by silica gel column chromatography, LH-20 column chromatography, silica gel TLC, and preparative HPLC. The molecular weight of sulfomycin Ia was determined to be m/z 1129 (M+Na)$^{+}$ by FAB mass measurement and $^{1}$H NMR spectroscopic analysis. The structure was assigned as a derivative of sulfomycin I with thiazole, methyloxazole, oxazole, and pyridine rings by $^{1}$H NMR spectral data.

Keywords

TipA promoter inducing compound;Screening;Chemical structure;Thiopeptide

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