알칼리 내성 Bacillus sp. YA-14 유래의 중복 Promotor를 이용한 재조합 Plasmid로부터의 Pectate Iyase의 발현

  • Park, Hee-Kyoung (Department of Biotechnology and Bioproduct Research Center, Yonsei University) ;
  • Hahm, Byoung-Kwon (Department of Food engineering, Dankook University, Korea and Bioproduct Research Center Yonsei University) ;
  • Yu, Ju-Hyun (Department of Biotechnology and Bioproduct Research Center, Yonsei University) ;
  • Bai, Dong-Hoon (Department of Food engineering, Dankook University, Korea and Bioproduct Research Center Yonsei University)
  • 박희경 (연세대학교 생명공학과.생물산업소재 연구센터) ;
  • 함병권 (단국대학교 식품공학과.연세대학교 생물산업소재 연구센터) ;
  • 유주현 (연세대학교 생명공학과.생물산업소재 연구센터) ;
  • 배동훈 (단국대학교 식품공학과.연세대학교 생물산업소재 연구센터)
  • Published : 1997.12.01

Abstract

For the overproduction of pectate lyase (PL), the recombinant plasmid pl2BS fl which has strong promoter from alkali-tolerent Bacillus sp. YA-14 was used. In order to overexpress the pectate lyase by the action of overlapping strong promoter in pl2BS$\Delta$fl, 1.6 kb of PL gene was inserted into pl2BS$\Delta$fl to form pl2BS$\delta$f1-PL and the enzyme was expressed. But decreased expression efficiency of the PL gene was observed and it was due to the presence of the transcription terminator region on the upstream of the PL gene. The transcription terminator of the PL gene in pl2BS$\delta$f1-PL was removed and the resulting plasmid p12BS$\Delta$fl$\Delta$PL was formed. Bacillus subtilis 207-25 harboring the recombinant plasmid, p12BS$\Delta$fl$\Delta$PL, revealed increased expression efficiency with chloramphenicol induction when cat-86 was used as a reporter gene.

Keywords

Alkali-tolerant Bacillus sp. YA-14;Strong promoter;Pectate lyase

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