Aspergillus niger에서 단백질분해효소 결함 돌연변이주의 제조 및 특성규명

  • Jeong, Heon Se ;
  • Chae, Suhn Kee ;
  • Park, Hee Moon ;
  • Maeng, Pil Jae ;
  • Kim, Jeong-Yoon
  • 정헌세 (충남대학교 미생물학과) ;
  • 채순기 (배재대학교 생화학과) ;
  • 박희문 (충남대학교 미생물학과) ;
  • 맹필재 (충남대학교 미생물학과) ;
  • 김정윤 (충남대학교 미생물학과)
  • Published : 1997.08.01


Several protease-deficient mutants of Aspergillus niger have been isolated by halo-screening on skim milk plate after UV irradiation of conidiospores. The extracellular proteolytic activities of the mutant strains grown in an optimized medium varied from 3% to 85% of that of the parental strain. Especially, two mutant strains named as ANPD-129 and ANPD-153, which had 3% and 49% of acid protease activity of the parental strain, respectively, were further characterized both physiologically and genetically. The growth rates of the mutants, ANPD-129 and ANPD-153, were similar to that of the parental strain, unlike other protease-deficient mutants. The diploid formed between the two mutants restored protease activity to a similar level of that of the parental strain. This result revealed that ANPD-129 and ANPD-153 had mutations at different loci. Using master strains with marked chromosomes these loci were assigned to linkage groups. The mutation locus (prt129) in ANPD-129 was assigned to linkage group VI and the locus (prt153) in ANPD-153 to linkage group III.


Aspergillus niger;Protease-dificient mutant;Chromosome mapping


  1. J. Gen. Microbiol. v.139 Regulation of secreted protein production by filamentous fungi: recent development and perspectives MacKenzie, D. A.;D. J. Jeenes;N. J. Belshaw;D. B. Archer
  2. Gene v.125 Cloning and characterisation of pepC, a gene encoding a serine protease from Aspergillus niger Frederick, G. D.;P. Rombouts;F. P. Buxton
  3. Gene v.145 Cloning and characterization of the pepE gene of Aspergillus niger encoding a new aspartic protease and regulation of pepE and pepC Jarai, G.;H. van den Hombergh;F. P. Buxton
  4. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O. H.;N. J. Rosebrough;A. L. Farr;R. J. Randall
  5. Curr. Genet. v.23 Genetic maps of eight linkage groups of Aspergillus niger based on mitotic mapping Debets, F.;K. Swart;R. F. Hoekstra;C. J. Bos
  6. FEMS Microbiol. Lett. v.107 A truncated glucoamylase gene fusion for heterologous protein secretion from Aspergillus niger Jeenes, D. J.;B. Marczinke;D. A. MacKenzine;D. B. Archer
  7. TIBTECH v.9 Mycelial morphology and metabolite production Braun, S.;S. E. Vecht-Lifshitz
  8. Bio/Technology v.8 Improved production of chymosin in Aspergillus by expression as a glucoamylase-chymosin fusion Ward, M. L.;Y. Wilson;K. H. Kodama;M. W. Rey;R. M. Berka
  9. More gene manipulations in fungi Heterologous gene expression in filamentous fungi Van den Hondel, C. A. M. J. J.;P. J. Punt;R. F. M. van Gorcom;J. W. Bennett(ed.);L. L. Lasure(ed.)
  10. Gene v.122 Heterologous gene expression in Aspergillus niger: a glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme Roberts, I. N.;D. J. Jeenes;D. A. MacKenzie;A. P. Wilkinson;I. G. Sumner;D. B. Archer
  11. Mol. Gen. Genet. v.234 Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases Mattern, I. E.;J. M. van Noort;P. van den Berg;D. Archer;I. N. Boberts;C. A. M. J. J. van den Hondel
  12. Appl. Microbiol. Biotechnol. v.43 Optimization of glucose oxidase production by Aspergillus niger using genetic- and process-engineering techniques Hellmuth, K.;S. Pluschkell;J. K. Jung;E. Ruttkowski;U. Rinas
  13. Gene v.132 Heterologous protein secretion directed by a repressible acid phosphatase system of Aspergillus niger MacRae, W. D.;F. P. Buxton;D. I. Gwynne;R. W. Davies
  14. J. Biol. Chem. v.266 The gene and deduced protein sequences of the zymogen of Aspergillus niger acid proteinase A Inoue, H.;T. Kimura;O. Makabe;K. Takahashi
  15. Presented at the 18th Fungal Genetic Conference Van den Hombergh, J. P. T. W.;G. Jarai;F. P. Buxton;J. Visser
  16. TIBTECH v.12 Protein secretion in filamentous fungi-trying to understand a highly productive black box Peberdy, J. F.
  17. Gene v.139 Cloning and characterization of the pepD gene of Aspergillus inger which codes for a subtilisin-like protease Jarai, G.;D. Kirchherr;F. P. Buxton
  18. Appl. Microbiol. Biotechnol. v.38 High level expression of the synthetic human lysozyme gene in Aspergillus oryzae Tsuchiya, K.;S. Tada;K. Gomi;K. Kitamoto;C. Kumagai;Y. Jigami;G. Tamura
  19. Bio/Technology v.5 Secretion of active human tissue plasminogen activator from the filamentous fungus Aspergillus nidulans Upshall, A.;A. A. Kumar;M. C. Bailey;M. D. Parker;M. A. Favreau;K. P. Lewison;M. L. Joseph;J. M. Maraganore;G. L. McKnight
  20. Appl. Microbiol. Biotechnol. v.38 Linkage group analysis in Aspergillus niger Bos, C. J.;S. M. Slakhorst;A. J. M. Debets;K. Swart
  21. Appl. Environ. Microbiol. v.62 Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains Gouka, R. J.;P. J. Punt;J. G. Hessing;C. A. van den Hondel
  22. Enzyme Microb. Technol. v.16 Strain improvement of chymosin-producing strains of Aspergillus niger var. awamori using parasexual recombination Bodie, E. A.;G. L. Armstrong;N. S. Dunn-Coleman
  23. Gene v.151 Cloning, characterization and expression of pepF, a gene encoding a serine carboxypeptidase from Aspergillus niger Van den Hombergh, J. P. T. W.;G. Jarai;F. P. Buxton;J. Visser
  24. Gene v.122 An inducible expression system for the production of human lactoferrin in Aspergillus nidulans Ward, P. P.;G. S. May;D. R. Headon;O. M. Conneely