Cellulose 분해효소를 분비하는 Trichoderma sp. C-4 균주의 분리 및 특성

  • Son, Young-June ;
  • Sul, Ok-Ju ;
  • Chung, Dae-Kyun ;
  • Han, In-Seob ;
  • Choi, Yun-Jae ;
  • Jeong, Choon-Soo
  • 손영준 (울산대학교 미생물학과) ;
  • 설옥주 (울산대학교 미생물학과) ;
  • 정대균 (경희대학교 유전공학과 및 유전공학연구소) ;
  • 한인섭 (울산대학교 미생물학과) ;
  • 최윤재 (서울대학교 동물자원학과) ;
  • 정춘수 (울산대학교 미생물학과)
  • Published : 1997.08.01

Abstract

During the screening of cellulase producing microorganisms, a fungal strain C-4 was selected from etiolated leaves. Based on taxonomic studies, the fungus could be classified as a strain of Trichoderma sp. When the strain C-4 was cultured in Mandels' media at 28$circ$C for 6 days, the enzyme activities detected in broth were as follows: 8.2 U/ml (28.1 U/mg) of CMCase activity, 0.75 U/ml (2.58 U/mg) of Avicelase activity, 1.67 U/ ml (5.68 U/mg) of $eta$-glucosidase activity. The optimum pH for enzyme induction was 6.2. The crude enzyme retained 100% of its original CMCase activity at 50$circ$C for 1 hr (pH 5.0), and at 4$circ$C for 24 hrs (pH 5.0). There was no effect on the CMCase activity in the presence of 1 mM of CsCl, LiCl, MgCl$_{2}$, and FeCl$_{2}$, respectively. When the crude enzyme was treated with trypsin and chymotrypsin (2% W/w) for 10 minutes, the remaining CMCase activity was 70%, but there was no further loss of activity for 60 minutes treatment at 30$circ$C. The crude enzyme showed the synergism with rumen fluid for the hydrolysis of Avicel and CMC by 118% and 130%, respectively.

Keywords

Trichoderma sp;Isolation;Identification;Cellulases

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