Botryococcus sp.의 성장에 미치는 광도와 영양염류의 영향

  • Oh, Hee-Mock ;
  • Kim, Seong-Bin ;
  • Park, Jun-Hong ;
  • Park, Eung-Roh ;
  • Lee, Sung-Taik ;
  • Kwon, Gi-Seok ;
  • Yoon, Byung-Dae
  • 오희목 (생명공학연구소 환경미생물RU) ;
  • 김성빈 (생명공학연구소 환경미생물RU) ;
  • 박준홍 (생명공학연구소 환경미생물RU) ;
  • 박응로 (한국과학기술원 생물과학과) ;
  • 이성택 (한국과학기술원 생물과학과) ;
  • 권기석 (생명공학연구소 환경미생물RU) ;
  • 윤병대 (생명공학연구소 환경미생물RU)
  • Published : 1997.08.01


Under the light intensity of 25-72$\mu$E/ m$^{2}$/s Botryococcus braunii UTEX 572 grew faster than Botryococcus sp. GE 24 isolated from a freshwater lake. The specific growth rate ($\mu$) of B. braunii UTEX 572 was highest at 0.260 (1/day) on a dry weight basis in Chu 13 medium from 1 to 9 days of incubation and then continuously decreased. Carbohydrate concentration and cellular nitrogen and phosphorus contents of B. braunii UTEX 572 gradually decreased with light intensity over a range of 25-72$\mu$E/m$^{2}$2/s, whereas the concentrations of protein and cellular N:P ratio increased with light intensity. Chlorophyll-a concentration showed a decreasing tendency with light intensity. The dry weight of B. braunii UTEX 572 increased in the highest rate of 83 mg/l/day at pH 8.0. When the N: P ratio of Chu 13 medium was adjusted to 50: 1 by addition of nitrogen source, dry weight increasing rate was 115 mg/l/day between 20 and 28 days of incubation which was the highest value during the cultivation. Cell growth in an open culture of B. braunii UTEX 572 was highest with Chu 13 medium, whereas that with Chu 13 medium adjusted to pH 7.0, containing 250 mg/l penicillin, or containing 1% glucose was reduced on a large scale. However, this result shows the possibility of the mass cultivation of B. braunii UTEX 572 in an open system competing with other microorganisms.


Botryococcus sp. Cell growth;Light intensity;Nitrogen;Phosphorus


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