Botryococcus sp.의 성장에 미치는 광도와 영양염류의 영향

  • Oh, Hee-Mock ;
  • Kim, Seong-Bin ;
  • Park, Jun-Hong ;
  • Park, Eung-Roh ;
  • Lee, Sung-Taik ;
  • Kwon, Gi-Seok ;
  • Yoon, Byung-Dae
  • 오희목 (생명공학연구소 환경미생물RU) ;
  • 김성빈 (생명공학연구소 환경미생물RU) ;
  • 박준홍 (생명공학연구소 환경미생물RU) ;
  • 박응로 (한국과학기술원 생물과학과) ;
  • 이성택 (한국과학기술원 생물과학과) ;
  • 권기석 (생명공학연구소 환경미생물RU) ;
  • 윤병대 (생명공학연구소 환경미생물RU)
  • Published : 1997.08.01

Abstract

Under the light intensity of 25-72$\mu$E/ m$^{2}$/s Botryococcus braunii UTEX 572 grew faster than Botryococcus sp. GE 24 isolated from a freshwater lake. The specific growth rate ($\mu$) of B. braunii UTEX 572 was highest at 0.260 (1/day) on a dry weight basis in Chu 13 medium from 1 to 9 days of incubation and then continuously decreased. Carbohydrate concentration and cellular nitrogen and phosphorus contents of B. braunii UTEX 572 gradually decreased with light intensity over a range of 25-72$\mu$E/m$^{2}$2/s, whereas the concentrations of protein and cellular N:P ratio increased with light intensity. Chlorophyll-a concentration showed a decreasing tendency with light intensity. The dry weight of B. braunii UTEX 572 increased in the highest rate of 83 mg/l/day at pH 8.0. When the N: P ratio of Chu 13 medium was adjusted to 50: 1 by addition of nitrogen source, dry weight increasing rate was 115 mg/l/day between 20 and 28 days of incubation which was the highest value during the cultivation. Cell growth in an open culture of B. braunii UTEX 572 was highest with Chu 13 medium, whereas that with Chu 13 medium adjusted to pH 7.0, containing 250 mg/l penicillin, or containing 1% glucose was reduced on a large scale. However, this result shows the possibility of the mass cultivation of B. braunii UTEX 572 in an open system competing with other microorganisms.

Keywords

Botryococcus sp. Cell growth;Light intensity;Nitrogen;Phosphorus

References

  1. SERI Report Collection, screening and characterization of lipid producing microalgae: process during fiscal year 1987 Johansen, J. R.;P. R. Lemke;W. R. Barclay;N. J. Nagle
  2. J. Phycol. v.4 Simple conditions for the growth of unicellular blue-green algae on plates Allen, M. M.
  3. Limnol. Oceanogr. v.22 Determination of total nitrogen in aqueous samples using persulfate digestion D'Elia, C. F.;P. A. Steudler;N. Corwin
  4. Appl. Microbiol. Biotechnol. v.29 Photosynthesis, growth and hydrocarbon production of Botryococcus braunii immobilized by entrapment and adsorption in polyurethane foams Bailiez, C.;C. Largeau;E. Casadevall;L. W. Yang;C. Berkaloff
  5. J. Appl. Phycol. v.3 A comparative study of microalgae isolated from flooded rice paddies: light-limited growth, C fixation, growth efficiency and relative N and P requirement Oh, H.-M.;G-Y. Rhee
  6. Phytochemistry v.26 Growth and production of cell constituents in batch cultures of Botryococcus sudeticus Vazquez-Duhalt, R.;H. Greppin
  7. FEMS Microbiol. Rev. v.16 Liquid and gaseous fuels from biotechnology: challenge and opportunities Kosaric, N.;J. Velikonja
  8. Botryococcus braunii. Agric. Biol. Chem. v.51 Lipid composition of a green alga Yamaguchi, K.;H. Nakano;M. Murakami;S. Konosu;O. Nakayama;M. Kanda;A. Nakamura;H. Iwamoto
  9. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O. H.;N. J. Rosebrough;A. F. Farr;R. J. Randall
  10. J. Phycol. v.21 Chemical profile of selected species of microalgae with emphasis on lipids Ben-Amotz, A.;T. G. Tornabene;W. H. Thomas
  11. J. Phycol. v.21 Growth and branched hydrocarbon production in a strain of Botryococcus braunii (Chlorophyta) Wolf, F. R.;A. M. Nonomura;J. A. Bassham
  12. Analyt. Chim. Acta v.27 A modified single solution method for the determination of phosphate in natural waters Murphy, J.;J. P. Riley
  13. Standard Methods for the Examination of Water and Wastewater(18th ed) APHA
  14. Biotechnol. Bioeng. v.27 Studies on batch and continuous cultures of Botryococcus braunii: Hydrocarbon production in relation to physiological state, cel ultrastructure, and phosphate nutrition Casadevall, E.;D. Dif;C. Largeau;C. Gudin;D. Chaumont;O. Desanti
  15. American Scientist v.46 The biological control of chemical factors in the environment Redfield, A. C.
  16. Limnol. Oceanogr. v.10 The measurement of total phosphorus in seawater based on the liberation of organically bound fraction of persulfate oxidation Menzel, D. W.;N. Corwin
  17. Enzyme Microb. Technol. v.16 Influence of nitrogen source and photoperiod on exopolysaccharide synthesis by the microalga Botryococcus braunii UC 58 Lupi, F. M.;H. M. L. Fernandes;M. M. Tome;I. Sa-Correia;J. M. Novais
  18. Korean J. Phycol. v.5 Preparation of unialgal cultures from natural waters by a micropipette technique Oh, H.-M.;G-Y. Rhee
  19. J. Appl. Phycol. v.2 Effects of nutrient and light on the biochemical composition of phytoplankton Harrison, P. J.;P. A. Thompson;G. S. Calderwood
  20. Biotechnol. Bioeng. v.22 Study of a Bloom of the oil-rich alga Botryococcus braunii in the Darwin River Reservoir Wake, L. V.;L. W. Hillen
  21. Limnol. Oceanogr. v.26 The effect of environmental factors on phytoplankton growth: Light and the interactions of light with nitrate limitation Rhee, G-Y.;I. J. Gotham
  22. Crit. Rev. Biotechnol. v.4 Microalgaculture Richmond, A. E.
  23. Appl. Biochem. Biotech. v.8 Botryococcus braunii: an unusual hydrocarbon-producing alga Wolf, F. R.
  24. Appl. Biochem. Biotech. v.11 Studies on the nutritional requirements of the oil-producing alga Botryococcus braunii Weetall, H. H.
  25. J. Plankton Res. v.8 Effects of light intensity and quality on the relative N and P requirement (the optimum N:P ratio) of marine planktonic algae Wynne, D.;G-Y. Rhee
  26. Experientia v.38 The production of hydrocarbons by Botryococcus braunii Bachofen, R.
  27. Anal. Chem. v.28 Colorimetric method for determination of sugars and related substances Dubois, M.;K. A. Gilles;J. K. Hamilton;P. A. Rebers;F. Smith