Candida tropicalis DS-72에 의한 Xylose로부터 Xylitol의 생산

  • 오덕근 (우석대학교 식품공학과 Tel. 82-652-290-1632 E-mail : deokkum@unitel.co.kr) ;
  • 김상용 (동양제과(주) 기술개발연구소)
  • Published : 1997.06.01

Abstract

A high xylitol producing yeast was isolated from the sludge of xylose manufacturing factory and then identified as Candida tropicalis DS-72 according to physiological properties. The strain was able to produce xylitol in a high concentration up to 72g/l from 100g/l xylose in 32 hours. Medium optimization for xylitol production by C. tropicalis DS-72 was performed. Effect of various nitrogen sources on xylitol production was investigated. Of nitrogenous compounds, yeast extract was the most suitable organic nitrogen nutrient for the enhancement of xylitol production. However, inorganic nitrogen resulted in a low cell concentration and did not produce xylitol. Effect of inorganic salts such as KH$_{2}$PO$_{4}$, and MgSO$_{4}$, 7H$_{2}$O on xylitol production was also studied. Optimal medium was selected as xylose 100g/l, yeast extract 10g/l, KH$_{2}$PO$_{4}$, 5 g/l and MgSO$_{4}$, 7H$_{2}$O 0.2 g/l. Xylitol of 88 g/l was produced from 100 g/l xylose in 30 hours using the optimal medium in a flask. In a fermentor, a fed-batch culture with 300g/l xylose was carried out. A final xylitol concentration of 240 g/l in the culture could be obtained in 43 hours of culture time by maintaining the high level of dissolved oxygen during growth phase and limiting the dissolved oxygen in the same culture during production phase. This result corresponded to a xylitol yield of 80% and a xylitol productivity of 5.58 g/1-h.

References

  1. J. Food Sci. v.45 A quantitative study of mannitol, sorbitol, xylitol, and xylose in wild berries and commercial fruits Makinen, K. K.;E. Soderling
  2. Food Technol. v.42 Xylitol in sugar-free confections Pepper, T.;P. M. Olinger
  3. Food Technol. v.32 Xylitol. Its properties and food applications Emodi, A.
  4. Adv. Food. Res. v.28 Food technological evaluation of xylitol Hyvonen, L.;P. Koivistoinen
  5. Biotechnol. Bioeng. v.25 Conversion of pentoses by yeasts Gong, C. S.;T. A. Claypool;L. D. McCracken;C. M. Maun;P. P. Ueng;G. T. Tsao
  6. Biotechnol. Bioeng. v.40 Production of xylitol from D-xylose by Candida tropicalis. Opotimization of production rate Horitsu, H.;Y. Yahashi;K. Takamizawa;K. Kawai;T. Suzuki;N. Watanabe
  7. BIotechnol. Lett. v.13 Xylitol production by Candida guillermondii. Fermentation behavior Meyrial, V.;J. P. Delgenes;R. Moletta;J. M. Navarro
  8. J. Ferment. Bioeng. v.67 Conversion of D-xylose into xylitol by immobilized cells of Candida pelliculosa and Methanobacterium sp. HU. Nishio, N.;K. Sugawa;N. Hayase;S. Nagai
  9. Biotechnol. Lett. v.11 D-xylose fermentation by Candida shehatae and Pichia stipitis at low dissolved oxygen levels in fed-batch cultures Du Preez, J. C.;B. van Driessel;B. A. Prior
  10. Agric. Biol. Chem. v.40 Xylitol production by Enterobacter liquefaciens Yoshitake, J.;M. Shimamura;H. Ishizaki;Y. Irie
  11. Agric. Biol. Chem. v.35 Production of polyalcohol by a Cornyebacterium sp., Part Ⅰ. Production of pentitol from aldopentose Yoshitake, J.;H. Ohiwa;M. Shimamura
  12. J. Ferment. Technol. v.66 Production of xylitol from D-xylulose by Mycobacterium smegmatis Izumori, K.;K. Tuzaki
  13. 산업미생물학회지 v.24 Candida parapsilosis 돌연변이주에의한 xylitol 생산시 배지조건의 최적화 오덕근;윤상현;김정민;김상용;김정회
  14. The yeasts (a taxonomic study) Kreger-Van Rij, N. J. W.
  15. Yeasts: Characteristics and identification Barnett, J. A.;R. W. Payne;D. Yarrow
  16. Enzyme Microb. Technol. v.16 Biochemistry and physiology of xylose fermentation by yeasts Hahn-Hagerdal, B.;H. Jeppsson;K. Skoog;B. A. Prior
  17. Biotechnol. Lett. v.16 Influence of magnesium concentration on growth, ethanol, xylitol production by Pichia stipitis NRRL Y-7124 Mahler, G. F.;D. V. Guebel
  18. J. Ferment. Bioeng. v.83 Improvement of xylitol production by controlling oxygen supply in Candida parapsilosis Kim, S. Y.;J. H. Kim;D. K. Oh
  19. 한국식품과학회지 v.28 Candida parapsilosis에 의한 xylitol 생산시 균체농도가 미치는 영향 김상용;윤상현;김정민;오덕근
  20. Biotechnol. Lett. v.11 D-xylose fermentation by Candida shehatae and Pichia stipitis at low dissolved oxygen levels in fed-batch cultures Du Preez, J. C.;B. van Driessel;B. A. Prior
  21. Biotechnol. Lett. v.13 Study of xylitol formation under oxygen limiting conditions Furlan, S. A.;P. Bouilloud;P. Strehaiano;J. P. Riba
  22. Biotechnol. Lett. v.17 Effect of nitrogen sources on xylitol production from D-xylose by Candida sp. L-102 Lu, J.;L. B. Tsai;C. S. Gong;G. T. Tsao
  23. J. Ferment. Bioeng. v.81 Production of xylitol from D-xylose by Candida tropicalis. The effect of D-glucose feeding Yahashi, Y.;H. Horitsu;K. Kawai;T. Suzuki;K. Takamizawa