CheY-OmpR Hybrid Protein Acting on the Osmoregulatory System

CheY-OmpR 혼성 단백질의 삼투조절효과

  • 고민수 (한국과학기술원 생물과학과) ;
  • 박찬규 (한국과학기술원 생물과학과)
  • Published : 1997.06.01

Abstract

In the previous study(6), we constructed the CheY-OmpR hybrid, Chp, which affects the expressions of ompF and om pC genes. Here we further characterize these effects and present the regulatory mechanism based on in vivo and in vitro data. Although Chp retained the sequence-specific DNA-binding ability, it was not possible to enhance transcriptional activity, suggesting that it may act as a competitive inhibitor to OmpR. The DNA-binding affinity of Chp was not modulated by phosphorylation of its Che Y portion. Chp was able to increase ompR transcription. FurthemlOre, it was found that the wild-type OmpR also exerts the same effect, which is also eOlltrolled by changes in medium osmolarity and in EnvZ activity.

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