CheY-OmpR Hybrid Protein Acting on the Osmoregulatory System

CheY-OmpR 혼성 단백질의 삼투조절효과

  • 고민수 (한국과학기술원 생물과학과) ;
  • 박찬규 (한국과학기술원 생물과학과)
  • Published : 1997.06.01


In the previous study(6), we constructed the CheY-OmpR hybrid, Chp, which affects the expressions of ompF and om pC genes. Here we further characterize these effects and present the regulatory mechanism based on in vivo and in vitro data. Although Chp retained the sequence-specific DNA-binding ability, it was not possible to enhance transcriptional activity, suggesting that it may act as a competitive inhibitor to OmpR. The DNA-binding affinity of Chp was not modulated by phosphorylation of its Che Y portion. Chp was able to increase ompR transcription. FurthemlOre, it was found that the wild-type OmpR also exerts the same effect, which is also eOlltrolled by changes in medium osmolarity and in EnvZ activity.


  1. Ann. Rev. Biochem. v.60 Signal transduction pathways involving proteins phosphorylation in prokaryotes Bourret, R. B.;K. A. Bordovich;M. I. Simon
  2. Mol. Microbiol. v.6 A novel sensor-regulator protein that belongs to the homologous family of signal-tranduction proteins involved in adaptive responses in Escherichia coli Nagasawa, S.;S. Tokishita;H. Aiba;T. Mizuno
  3. J. Biol. Chem. v.264 Identification of the site of phosphorylation of the chemotaxis response regulator proteins, Che Y Sanders, D. A.;B. L. Gillece-Castro;A. M. Stock;A. L. Burlingame;D. E. Koshland, Jr.
  4. J. Biol. Chem. v.266 Roles of the highly conserved aspartate and lysine residues in the response regulator of bacterial chemotaxis Lukat, G. S.;B. H. Lee;J. M. Mottonen;A. M. Stock;J. B. Scock
  5. Proc. Natl. Acad. Sci. USA v.81 A uninque mechanism regulating gene expression: Translational inhibition by a complementary RNA transcrip(micRNA) Mizuno, T.;M. Chou;M. Inouye
  6. J. Bacteriol. v.174 Positive and negative control of ompB transcription on Escherichia coli by cyclic AMP and the cyclic AMP receptor protein Huang, L.;P. Tsui;M. Freundllich
  7. Cell. v.49 Conserved domains in bacterial regulatory proteins that respond to environmental stimuli Ronson, C. W.;B. T. Nixon;F. Ausubel
  8. J. Biol. Chem. v.264 Identification of the DNA-binding domain of the OmpR protein requred for transcriptional activation of the ompF and ompC genes of Escherichia coli by in vivo DNA footprinting Tsung, K.;R. E. Brissette;M. Inouye
  9. Chem. Rev. v.87 Molecular components of bacterial chemotaxis Stewart, R. C.;F. W. Dahlquist
  10. J. Bacteriol. v.176 A distant upstream site involved in the negative regulation of the Escherichia coili ompF gene Huang, K.;J. L. Schieberl;M. M. Igo
  11. Science. v.245 Activation of bacterial porin gene expression by a chimeric signal transducer in response to aspartate Utsumi, R.;R. E. Brissette;A. Rampersaud;S. A. Forst;K. Osoawa;M. Inouye
  12. Proc. Natl. Acad. Sci. v.81 Phosphorylation of bacterial response refulator proteins by low molecular weight phospho-donors Lukat, G. S.;W. R. McCleary;A. M. Stock;J. B. Stock
  13. Kor. J. Genetics. v.15 no.1 CheY-OmpB Chimeric Response Refulator Ko, M;C. Park
  14. Proc. Natl. Acad. Sci. v.85 Transmitter and receiver modules in bacterial signalin gproteins Kofoid, E. C.;J. S. Parkinson
  15. Experiments in molecular genetics. Miller, J. H.
  16. J. Mol. Biol. v.222 EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes Russo, F. D.;T. J. Silhavy
  17. Cell. v.52 A physiological role for DNA supercoling in the osmotic regulation of gene expression in S. typhimurium and E. Coli Higgins, C. F.;C. J. Dorman;D. A. Striling;L. Waddell;I. R. Booth;G. May;E. Bremer
  18. J. Mol. Biol. v.243 OmpR mutants specifically degective for transcriptional activation Pratt, L. A.;T. J. Silhavy
  19. J. Mol. Biol. v.146 The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12 Hall, M. N.;T. J. Silhavy
  20. J. Biol. Chem. v.157 Osmoregulation of the gene expression: I. DNA sequence of the ompR gene of the ompB operon of Escherichia coli and characterization of its gene producet Wurzel, E. T.;M. Y. Chou;M. Inouye
  21. Ann. Rev. Genetics. v.26 Communication modules in bacterial signaling proteins Parkinson, J. S.;E. C. Kofoid
  22. J. Biol. Che. v.263-2 Internation of OmpR, a positive regulator, with the osmoregulated ompC and ompF genes of Escherichia coli Mizuno, T.;M. Kato;Youl-Lae Jo;S. Mizushima
  23. J. Bacteriol. v.173 cis-Acting ompF Mutations that result in OmpR-dependent constitutive espression Slauch, J. M.;T. J. Silhavy
  24. J. Bacteriol. v.168 Transcriptional control of flagellar genes in Escherichia coli K-12 Komeda, Y.