Interaction of Enrofioxacin-Colistin Combination and LPS-Neutralization of the Different Antibiotic Classes In Vitro

시험관내에서 Enrofloxacin과 Colistin의 병용투여시 상호작용과 항균물질들의 독소중화능

  • 박승춘 (한국과학기술원 생명공학연구소) ;
  • 김민규 (충남대학교 수의과대학 약리독성학실험실) ;
  • 윤효인 (충남대학교 수의과대학 약리독성학실험실) ;
  • 오태광 (한국과학기술원 생명공학연구소)
  • Published : 1997.06.01


In the present study, we investigated the neutralization activity of various antimicrobials against lipopolysaccharide (LPS) as well as interaction between two antimicrobials (enrofioxacin and coilstin) using checkerboard method. The neutralization activity of antimicrobials used in the test was assayed by means of LAL chromogenic test after reaction of LPS with colistin, enorfioxacin, ampicillin, polymyxin B, oxytetracycline, streptomycin, and erythromycin. As the results, the neutralization activity of coltstin and polymixin B had a more stronger than that of tested other antimicrobials. In bacterial culture broth, the best neutralization activity of the antibiotics was also shown to coltstin and polymixin B. Meanwhile, It was shown to have synergism between enorfloxacin and coltstin on the basts of FIC (fractional inhibition concentration). The FIC of enorfioxacin-colistin combinations was 0.50-1.03 to Staphylococcus aureus R-209, 1.03-1.06 to Salmonella typhimurium, 0.75-1.25 to Bordetella Bronchtseptica and 1.02-1.25 to E. coli K88ab. On the basts of the above results, the present study may be of clinical usefulness in the choice of an antibiotic therapy for severe sepsts in animals.


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