Effect of Matruation Media and Liquid Boar Semen on Maturation and Fertilization of Pig Oocytes In Vitro

돼지 난모세포의 체외성숙 및 체외수정시 배양액과 액상정액의 효과

  • Park, C.S. (College of Agriculture, Chungnam National University) ;
  • Lee, K.S. (College of Agriculture, Chungnam National University) ;
  • Park, B.K. (College of Agriculture, Chungnam National University) ;
  • Zhang, X.K. (Tian Jin Institute of Animal Husbandry and Veterinary Science) ;
  • Lee, Y.H. (Tian Jin Institute of Animal Husbandry and Veterinary Science) ;
  • Xu, Z. (Tian Jin Institute of Animal Husbandry and Veterinary Science)
  • Published : 1997.03.01

Abstract

This present study was carried out to examine the effect of maturation media and liquid boar semen on in vitro maturation and feritilization of pig oocytes. The results obtained were as follows : When the oocytes were cultured for 36∼42 hours in mTCM-199, Waymouth MB 725/1 and mTLP-PVA medium, the maturation rates were 90%, 92% and 88%, respectively. The sperm penetration rates of pig oocyte matured in vitro were 87%(mTCM-199), 90%(Waymouth MB 725/1) and 86%(mTLP-PVA), respectively. The rates of nuclear maturation and fertilization of pig oocytes among three different media did not differ. However, the rate of male pronucleus formation of pig oocytes was significantly higher in pig oocytes matured in Waymouth MB 725/1(91%) than oocytes matured in mTCM-199(66%) and mTLP-PVA(62%) medium (P<0.05). When the collected sperm-rich fraction without diluent was used fro in vitro fertilization in mTCM-199 fertilization medium, the fertilization rate was 87.9%. However, when the liquid boar semen diluted with B tschwiler diluent was used at day 3 and 5 after dilution, the fertilization rate was 40.8% and 0.0%, respectively.