Binding Affinity between Lectin and Rhizobia in Soybean-Bradyrhizobium Symbiosis

대두-근류균의 공생에서 Lectin에 의한 결합특이성

  • Kim, Jin-Ho (Dept. of Agronomy, Sangju National Polytechnic University) ;
  • Park, Woo-Churl (Dept. of agricutural Chemistry, Kyungpook National University) ;
  • Kang, Sang-Jae (Dept. of agricutural Chemistry, Kyungpook National University)
  • Published : 1997.12.31

Abstract

This study was carried out to elucidate the biological characteristics of Rhizobia in biological nitrogen fixation system. The results of investigation were as follows; Polyacrylamide gel electrophoresis pattern of root lectin in the presence of SDS was ascertained electrophoretically and chromatographically. The purified root lectin formed immunoprecipitin line with anti lectin rabbit IgG. Root lectin, seed lectin and root exudate were tested for chemotactic ability. Chemotactic responses of RCR3407 and KCTC2422 toward root exudate were stronger than those of seed lectin and root lectin, but there didn't occur chemotactic responses of LPN100, not bound with seed lectin and that of LPN101, bound with seed lectin toward root exudate, root lectin and seed lectin. RCR3407, KCTC2422 and LPN-101, which nodulated with soybean, interacted with soybean lectin, but not with pea lectin. LPN-100, which was not nodulated with soybean, didn't interact with soybean lectin.