Effect of Neupectin-L on Ethanol Production from Raw Starch Using a Co-Immobilized Aspergillus awamori and Zymomonas mobilis

Aspergillus awamori와 Zymomonas mobilis로 구성된 혼합고정화 배양계의 에탄올 생산에 미치는 Neupectin-L의 영향

  • Lee, Sang-Won (Dept. of Food Science and Technology, Gyeongsang National University) ;
  • Cho, Yong-Un (Dept. of Microbiological Engineering, Chinju National University) ;
  • Kim, Hong-Chul (Dept. of Microbiological Engineering, Chinju National University) ;
  • Park, Seok-Kyu (Dept. of Food and Nutrition, Sunchon National University) ;
  • Sung, Nak-Kie (Dept. of Food Science and Technology, Gyeongsang National University)
  • 이상원 (경상대학교 식품공학과) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 김홍출 (진주산업대학교 미생물공학과) ;
  • 박석규 (순천대학교 식품영양학과) ;
  • 성낙계 (경상대학교 식품공학과)
  • Published : 1997.04.28

Abstract

In order to reduce energy input in direct ethanol production from raw starch by co-immobilized Aspergillus awamori(A) and Zymomonas mobilis(Z), A-Z 36 culture system which was changed to anaerobic after 36 h of aerobic fermentation without sterilization was investigated. This immobilized cell system can not be carried out under unsterile conditions because of growth of microbial contaminants from original medium. Among some food additives such as sorbic acid, benzoic acid, dehydroacetic acid, p-hydroxybenzoic acid, Vantocil IB and Neupectin-L, Vantocil IB and Neupectin-L were a potent antibacterial agent in A-Z 36 culture cell system and were not affected in hydrolysis of substrate as compared with the case of control. Ethanol yield(6.9 g/l) in system of addition of 0.1% Neupectin-L was slightly higher than that in control(6.4 g/l). When 2% starch was fed five times in fed-batch culture with 0.1% Neupectin-L, ethanol yield and productivity were 34 g/l and 2.0 g/l/day, respectively.