Enzymatic Characteristics for Xylanase Activity of Tremella fuciformis and its Symbiotic Fungi

흰목이버섯 및 공생균이 분비하는 Xylanase 효소적 특성

  • Chang, Hyun-You (Department of Industrial Crops, Korea National Agricultural College, RDA) ;
  • Kim, Gwang-Po (National Institute of Agricultural Science and Technology, RDA) ;
  • Hong, In-Pyo (National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Han-Kyoung (National Institute of Agricultural Science and Technology, RDA) ;
  • Chung, Jong-Cheon (National Institute of Agricultural Science and Technology, RDA) ;
  • Sung, Jae-Mo (Department of Agricultural Biology, Kangwon National University)
  • 장현유 (한국농업전문학교 특용작물과) ;
  • 김광포 (농업과학기술원 응용미생물과) ;
  • 홍인표 (농업과학기술원 응용미생물과) ;
  • 김한경 (농업과학기술원 응용미생물과) ;
  • 정종천 (농업과학기술원 응용미생물과) ;
  • 성재모 (강원대학교 생명자원과학대학 농생물학과)
  • Published : 1997.12.30

Abstract

Effects of cultural conditions on the production of xylanase by Tremella fuciformis, symbiotic fungi and mixed fungi were investigated. The optimum carbon source for high production of xylanase by T. fuciformis, symbiotic fungi and mixed fungi was xylose. The optimum nitrogen source for both T. fuciformis and symbiotic fungi was $KNO_3$, whereas mixed fungi was $(NH_4)_2SO_4$. The optimum culture period for high production of xylanase was 5 days for both T. fuciformis and mixed fungi, and 6 days for symbiotic fungi, respectively. The optimum temperature for T. fuciformis and symbiotic fungi was $40^{\circ}C$, and the corresponding value for mixed fungi was $45^{\circ}C$. Xylanase activity was high at pH 6 for T. fuciformis and symbiotic fungi, and pH 7 for mixed fungi. Except $Hg^{2+}$ and $Pb^{2+}$, metal ions in T. fuciformis inhibited the activity of xylanase, and, thermal stability of xylanase in T. fuciformis, symbiotic fungi and mixed fungi maintained 80% of activity until $50^{\circ}C$. The Michaelis constant (Km) of xylan was $6.25{\times}10^{-5}\;M$ in T. fuciformis, $5.6{\times}10^{-2}\;M$ in symbiotic fungi, $5.2{\times}10^{-2}\;M$ in mixed fungi.

References

  1. 日農化大會講演(IV) 高橋光雄
  2. 生物工學會誌 v.72 no.1 特異な不完全菌 Fusidium sp. Box-1の 生成する キシラナゼ 大野信子;藤原佳奈;條山浩文;騰井貴明
  3. 日農化大會講演(IV) 稻綱惠;早田尙
  4. 日農化大會講演(IV) 稻綱惠;早田尙
  5. 農化 v.34 Purifiation and properties of ${\beta}-1$,4'-xylanase from Chaetomium globusum $A_2$ 福井作藏;鈴木藏夫;北原覺雄;三輪知雄
  6. 日本菌學會大會講演要旨集 福佳俊郞
  7. 農業論文集 v.38 no.1 장수버섯 菌絲體 培養으로부터 xylanase 生産과 分離 張炫酉;車東烈;森永 力
  8. 農化誌 v.14 Myriococcum albomyces가 生産하는 cellulase에 關한 硏究 鄭東孝
  9. Kor. J. Appl. Microbiol. Bioeng. v.10 no.2 Studies on a-amylase of Bacillus circulans F-2. Part II. Enzymatic characteristics of the purified ${\alpha}-amylase$ Chung, M.J.;Taniguchi, H.;Maruyama, Y.;Lee, M.J.
  10. Appl. Environ. Microbiol. Purification and characterization of extracellular amylolytic enzymes from the Yeast. Filobasidium capsuligenum De Mot, R.;Verachtert, H.
  11. Int. Biodetn. Bull. v.7 Purification and characteristics of xylanase from the rot fugus Streum sanguinolentum Ericksson, K.E.;Pettersson, B.
  12. ${\alpha}-amylase$;the Enzymes, 4 Fisher, E.H.;E. Stein, A.
  13. Developments in microbial extracellular enzymes;topics enzyme and fermentation biotechnology, 3 Forgarty, W.M.;Kelly, C.T.
  14. J. Gen. Appl. Microbiol. v.4 Purification and some properities of Aspergillus batae Fukui, S.
  15. Cultivation of Tremella Huang, N.L.
  16. Biochem. J. v.108 The xylanase system of Coniophora cerebella King, N.J.;Fuller, D.B.
  17. Agr. Biol. Chem. v.39 no.7 A new method for preparation of xylobiose, eliminating xylose from enzymatic xylan hydrolate by yeast Kusakabe, I.;Yasui, T.;Kobayashi, T.
  18. J. Ferment. Technol. v.41 Studies on cellulotic enzymes produced by Aspergillus saitoi. Inhibition and activation of carboxymethyl cellulase (IV) Matsumura, C.;Maejima, K.
  19. J. Biol. Chem. v.153 Photometric adaptation of the Somogyi method for the determination of glucose Nelson, N.
  20. Kor. J. Appl. Microbial. Bioeng. v.9 no.2 Studies on the production of thermostable amylase Oh, D.H.;Lee, K.P.;Pyun, Y.R.;Yu, J.H.
  21. Appl. Environ. Microbiol. v.36 no.6 Production, characterization and partial amino acid sequence of xylanase from Schizophyllum commune Paice, M.G.;Jurasek, L.;Carpenter, M.R.;Smillie, L.B.
  22. Can. J. Microbiol. v.2 Production of xylanase from fungi Simpson, F.J.
  23. J. Biol. Chem. v.195 Notes in sugar determination Somogyi, M.
  24. J. Ferment. Technol. v.57 Effect of chemical treatment of solubilization of crystaline cellulose and cellulosic wastes with Pelliclaria filamentosa cellulose Tanaka, M.;Taniguchi, M.;Morita, T.;Matsumo, R.;Kamikubo, T.
  25. Appl. Environ. Microbiol. v.57 no.2 Purification and properties of ${\beta}-1$, 4-xylanase from Aeromonas caviae W-61 Viet, D.N.;Kamio, Y.;Abe, N.;Kaneko, J.;Izaki, K.