A Simple Method for Generation of Homologous Internal Standards for Competitive PCR

  • Choi, Eu-Na (Department of Biochemistry, College of Medicine, University of Ulsan) ;
  • Hahn, Sung-Sik (Department of Biochemistry, College of Medicine, University of Ulsan) ;
  • Choi, Kyung-Hee (Department of Biology, College of Natural Sciences, Chung-Ang University) ;
  • Na, Doe-Sun (Department of Biochemistry, College of Medicine, University of Ulsan)
  • Received : 1996.06.10
  • Published : 1996.09.30


In competitive PCR, which is used to quantify target DNA an internal standard is needed. Here we present a simple method to construct homologous competitive standards. The method, which is based upon deletion of a portion of the target DNA, does not require any additional primers. This is the simplest method developed thus far to construct a competitive standard. The whole procedure. from construction of a competitive standard to quantitation by PCR, can be completed within a single day.


competitive standard;PCR


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