Molecular Characterization of the Region Encoding Integrative Functions from Enterococcal Bacteriophage ${\phi}$FC1

  • Kim, Min-Jung (Doping Control Center, Korea Institute of Science and Technology) ;
  • Lee, Jin-Young (Molecular Design Laboratory, Hanhyo Institute of Technology) ;
  • Kim, Young-Woo (Department of Genetic Engineering, Korea University) ;
  • Sung, Ha-Chin (Department of Genetic Engineering, Korea University) ;
  • Chang, Hyo-Ihl (Department of Genetic Engineering, Korea University)
  • Received : 1996.05.17
  • Published : 1996.09.30

Abstract

Bacteriophage ${\phi}FC1$ is a temperate phage which was identified as a prophage in the Enterococcus faecalis KBL703 chromosome. Phage ${\phi}FC1$ integrates into the host chromosome by site-specific recombination. The phage attachment site P (attP) was localized within the 0.65-kb XhoI-HindIII fragment and the nucleotide sequence of the region was determined. An open reading frame (mj1) which adjoined the phage attachment site encoded a deduced protein related to the site-specific recombinase family. The organization of this region was comparable to other site-specific recombination systems. The molecular weight of the expressed MJ1 in E. coli was in good agreement with the predicted 53,537 Da of the mj1 gene product. Elucidation of the phage-specific integration process in this study would provide useful genetic tools such as a chromosomal integration system.

Keywords

attachment site P (attP);site-specific recombinase;site-specific recombinase family;site-specific recombination

References

  1. J. Infect. Dis. v.170 Olmsted, S.B.;Dunny, G.M.;Erlandsen, S.;Wells, C.L. https://doi.org/10.1093/infdis/170.6.1549
  2. Proc. Natl. Acad. Sci. USA v.80 Plasterik, R.H.A.;Ad Brinkman;Pieter van de Putte https://doi.org/10.1073/pnas.80.17.5355
  3. J. Bacteriol. v.174 Raya, R.R.;Fremaux, C.;de Antony, G.L.;Klaenhamer, T.R. https://doi.org/10.1128/jb.174.17.5584-5592.1992
  4. Molecular Cloning, A Laboratory Manual Sambrook, J.;Fritsch, E.F.;Maniatis, T.
  5. Proc. Natl. Acad. Sci. USA v.74 Sanger, F.;Nklen, S.;Coulson, A.R. https://doi.org/10.1073/pnas.74.12.5463
  6. J. Bacteriol. v.172 Sato, T.;Samori, Y.;Kobayashi, Y. https://doi.org/10.1128/jb.172.2.1092-1098.1990
  7. J. Bacteriol. v.171 Ye, Z.;Lee, C.Y. https://doi.org/10.1128/jb.171.8.4146-4153.1989
  8. EMBO J. v.5 Argos, P.Landy, A.;Abremski, K.;Egan, J.B.;Haggard-Ljungquist, E.;Hoess, R.H.;Kahn, M.L.;Kalionis, B.;Narayana, S.V.L.;Pierson, L.S. III;Sternberg, N.;Leong, J.M.
  9. Mobile Genetic Elements Campbell, A.;Shapiro, J.A.(ed.)
  10. Appl. Environ. Microbiol. v.57 Hill, C.;Massey, I.J.;Klaenhammer, T.R.
  11. Appl. Environ. Microbiol. v.53 Jos, B.M.Vossen, V.D.;Lelie, D.V.D.;Venema, G.
  12. Mol. Cells v.4 Kim, Y.W.;Chang, H.I.
  13. Annu. Rev. Biochem. v.58 Landy, A. https://doi.org/10.1146/annurev.bi.58.070189.004405