Factors Affecting HETP in Preparative Liquid Chromatography

제조용 액체 크로마토그래피에서 HETP에 영향을 미치는 인자

  • Choi, Du Chan (Dept. of Chem. Eng., Inha university) ;
  • Choi, Dai-Ki (CFC Alternatives Technology Center, Korea Institute of Science and Technology) ;
  • Row, Kyung Ho (Dept. of Chem. Eng., Inha university)
  • 최두찬 (인하대학교 화학공학과) ;
  • 최대기 (한국과학기술연구원, CFC 대체기술센터) ;
  • 노경호 (인하대학교 화학공학과)
  • Received : 1996.07.16
  • Accepted : 1996.09.09
  • Published : 1996.10.10

Abstract

In chromatographic separation, HETP(height equivalent to a theoretical plate) is a useful quanititive parameter and it is wildely designated as column efficiency. The effects of operating conditions (sample concentration, injection volume, flow rate and mobile phase composition) on HETP were investigated in perparative liquid chromatography (PLC). Water and organic modifier of methanol were used as mobile phase. The sample of thymidine was injected into preparative C18 columns. The system was run by a isocratic mode in 1.5~5.5ml/min. The larger amounts of sample and higher flow rates of mobile phase increased HETP, which means that column efficiencies were worse. As the weight of sample injected into a chromatographic system could be prepared with different concentrations and injection volumes, for the same amount of sample, HETP was approximately increased two times with the ten-fold injection volume. HETP was mainly affected by the resistance of stationary and mobile phase mass transfer in the intraparticle section of packings at higher velocities.

Keywords

Acknowledgement

Supported by : 인하대학교

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