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EFFLUENT FROM RUSITEC INOCULATED WITH RUMEN LIQUOR OR COW FAECES AS SOURCES OF MICRO-ORGANISMS FOR IN VITRO DIGESTION OF FORAGES

  • Akhter, S. ;
  • Owen, E. ;
  • Hossain, M.M.
  • Received : 1995.05.30
  • Accepted : 1996.02.05
  • Published : 1996.08.01

Abstract

The experiment investigated the possibility of using effluent from RUSITEC (rumen simulation technique) inoculated with rumen liquor or cow faeces as sources of micro-organisms for in vitro digestion of forages. Nine forages ${\times}3$ sources of inoculum were used in a factorial arrangement of treatments. Rumen liquor was collected from fistulated sheep and faeces was collected from cows. The RUSITEC apparatus consisted of 4 vessels, 2 vessels were charged with faecal liquor and 2 with rumen liquor. On the 8th day of the experiment RUSITEC effluent were collected to use in in vitro studies. In vitro OMD (g/kg) values using three sources of inoculum (fresh rumen liquor, RUSITEC effluent from rumen liquor or cow faeces) were statistically significant (p < 0.001). The regression relationships between OMD using fresh rumen liquor and RUSITEC effluent were highly significant ($R^2>0.90$). The results suggest that RUSITEC effluent either from rumen liquor or cow faeces can be used as a source of micro-organisms for in vitro digestion of forages.

Keywords

RUSITEC;Inoculum;In Vitro Digestion;Forages